纯钛表面涂层(Ca+Zn)/P比值与成骨细胞生物活性的关系  

Study of relationship between(Ca +Zn)/P of pure titanium surface coating and biological activity of osteoblasts

作　　者：商亚微[1] 张伟[2] 何晶[1] 王丹宁[1] 钟鸣[3] 赵宝红[1] 

机构地区：[1]中国医科大学附属口腔医院种植中心,辽宁省口腔医学研究所口腔种植研究室,辽宁省口腔疾病转化医学研究中心,辽宁沈阳110002 [2]中国科学院金属腐蚀与防护研究所,辽宁沈阳110016 [3]中国医科大学口腔医学院中心实验室,辽宁沈阳110002

出　　处：《上海口腔医学》2015年第1期46-51,共6页Shanghai Journal of Stomatology

基　　金：辽宁省自然科学基金(20092093);辽宁省科技计划项目(2013225049);沈阳市科技计划项目(F11-264-1-25)~~

摘　　要：目的：研究纯钛表面不同（Ca＋Zn）/P比值对成骨细胞（MC3T3-E1）黏附、增殖和分化的影响,确定纯钛表面生物活性最佳的Ca、P、Zn含量。方法：在微弧氧化的电解液中加入一定浓度的Ca、P和5种不同浓度的Zn（0、0.01、0.03、0.04和0.06 mol/L）,使Ca、P元素的摩尔比值接近羟基磷灰石的比值。在纯钛材料表面制备5种不同（Ca＋Zn）/P比值的生物活性涂层,分别记为S0、S1、S2、S3和S4组。应用XPS和SEM分析样品表面的元素组成、存在形式、（Ca＋Zn）/P比值以及表面形貌;应用SEM、MTT法和ALP活性测定法分析材料表面培养的MC3T3-E1细胞黏附、增殖和分化情况。采用SPSS13.0软件包对数据进行统计学分析。结果：随着电解液中Zn浓度的增加,S0~S4组（Ca＋Zn）/P比值分别为2.94、2.64、2.71、2.87和2.72,其中,在S3组时达到最大,S4组时降低。MC3T3-E1细胞在5组材料表面黏附、增殖和分化能力,依次为S3〉S4〉S2〉S1〉S0,各组之间差异具有显著性（P〈0.05）。结论 ：应用微弧氧化法在纯钛表面制备的不同含量Ca、P、Zn涂层,随着（Ca＋Zn）/P比值的增大,成骨细胞的黏附、增殖和分化能力随之增强。其中,（Ca＋Zn）/P比值为2.87时,细胞的生物活性最佳。PURPOSE： To study osteoblast（MC3T3-E1 cells） adhesion, proliferation and differentiation activity on the surface of pure titanium with different（Ca＋Zn）/P ratios,and determine Ca,P,Zn contents offering best biological activity on pure titanium surface. METHODS： In the micro-arc oxidation electrolyte, Ca, P with a certain concentration, and 5 different concentrations of Zn（0,0.01,0.03,0.04, and 0.06 mol/L） were added, which made the mole ratio of Ca and P element close to the ratio of hydroxyapatite.（Ca ＋Zn）/P ratio of bioactive coating on 5 different pure titanium material surfaces were prepared, which named S0 and S1, S2, S3 and S4group, respectively. The surface elements, existence form,（Ca＋ Zn）/P ratio and surface morphology were analyzed by XPS and SEM; MC3T3-E1 cells adhesion, proliferation and differentiation cultured on the material surface were analyzed by SEM, MTT method and ALP activity assay. RESULTS：The data was analyzed with SPSS13.0 software package. With the increase of Zn content in the electrolyte,（Ca＋Zn）/P ratios were 2.94, 2.64 2.71,2.87 and 2.72 in S0~ S4 group, respectively.（Ca＋Zn）/P ratio was maximal in S3 group and decreased in S4 group. MC3T3-E1 cell adhesion, proliferation and differentiation capacity on material surface of 5 groups changed in the following order： S3 S4 S2 S1 S0, with significant differences among 5 groups（P 0.05）. CONCLUSIONS：Coating with different concentrations of Ca, P, Zn on pure titanium surface by micro-arc oxidation technique, the adhesion,proliferation and differentiation ability of osteoblast increase as（Ca＋Zn）/P ratio increase, and cell biological activity is the best when the（Ca＋Zn）/P ratio reached 2.87.

关 键 词：微弧氧化 钛 锌 钙 磷 成骨细胞 

分 类 号：R783.6[医药卫生—口腔医学]