富血小板纤维蛋白对体外培养的成骨细胞生物学特性的影响  

The effect of platelet-rich fibrin on biologic characteristics of osteoblasts

作　　者：孙晓琳[1] 周延民[1] 赵静辉[1] 郑玲[1] 杨婷婷[1] 

机构地区：[1]吉林大学口腔医院口腔种植科,吉林长春130021

出　　处：《上海口腔医学》2015年第1期61-64,共4页Shanghai Journal of Stomatology

摘　　要：目的 :探讨富血小板纤维蛋白(platelet-rich fibrin,PRF)对成骨细胞生物学特性的影响。方法 :体外培养成骨细胞MG63,实验组采用PRF,对照组为正常高糖DMEM培养液。以MTT法检测细胞增殖,碱性磷酸酶(ALP)染色法检测ALP活性,免疫组织化学法检测Ⅰ型胶原、骨保护素(OPG)及其配体(RANKL)的表达。采用SPSS17.0软件包对数据进行统计学分析。结果:PRF能够促进细胞增殖、ALP分泌、Ⅰ型胶原和骨保护素的表达,对骨保护素配体的表达影响不明显。结论:PRF能够促进成骨细胞增殖、分化及骨保护素的表达,PRF可能通过对骨保护素及其配体的调节,参与骨的重建过程。PURPOSE： To investigate the effect of platelet-rich fibrin on biologic characteristics of osteoblasts.METHODS： Osteoblasts were cultured with or without a PRF membrane. The cell proliferation was detected by MTT. The alkaline phosphatase（ALP） stain and the positive expression of collagen type Ⅰ, osteoprotegerin and RANKL were measured. The data was statistically analyzed with SPSS17.0 software package. RESULTS： PRF promoted cell proliferation and increased expression of ALP, collagen type Ⅰand OPG significantly, but there was no significant impact on expression of RANKL. CONCLUSIONS： PRF promotes osteoblasts proliferation, differentiation and OPG expression.PRF is involved in bone remodeling via regulating expression of OPG and RANKL.

关 键 词：富血小板纤维蛋白 成骨细胞 骨保护素及其配体 

分 类 号：R782[医药卫生—口腔医学]