正交试验优选益气固脱颗粒醇提工艺研究  

Optimization of Alcohol Extraction Process of Yiqigutuo Granules by Orthogonal Test

作　　者：孙贤[1] 谈瑄忠[1] 郎永英 

机构地区：[1]南京市中医院药剂科,南京210001 [2]南京中医药大学药学院

出　　处：《中国药师》2015年第3期410-413,共4页China Pharmacist

基　　金："十二五"南京市医学科技发展重大项目(编号:ZKX10027)

摘　　要：目的:优选益气固脱颗粒醇提工艺。方法:以正交设计的试验方法,对组方中有效成分黄芪甲苷、阿魏酸用HPLC法进行定量分析,选取L9(34)正交表,考察乙醇浓度、乙醇用量、提取次数、提取时间等工艺参数对益气固脱颗粒提取量的影响。结果:方差分析表明,乙醇浓度与提取次数对浸出物的含量及阿魏酸的提取有显著影响。最佳醇提工艺即加10倍量的70%的乙醇,提取3次,每次1.5 h。结论:该工艺稳定可行,适于工业生产。Objective：To optimize the alcohol extraction process of Yiqigutuo granules. Methods： Using the L9 （34 ） orthogonal design method, the active ingredients astragaloside and ferulic acid in the formula were quantitatively analyzed by HPLC. The concen-tration and amount of ethanol, extraction times and extraction duration and the other parameters affecting the extraction volume were ex-amined. Results：Analysis of variance showed that the ethanol concentration and extraction times had significant influence on the ex-tract content and the extraction of ferulic acid. The best alcohol extraction process was as follows：70% ethanol of 10-fold volume, ex-tracting 3 times with 1. 5h for each time. Conclusion：The process is stable, and suitable for the industrial production.

关 键 词：黄芪甲苷 阿魏酸 醇提工艺 正交试验 

分 类 号：R284.2[医药卫生—中药学]