银杏内酯和白果内酯对大鼠脑缺血再灌注损伤后VEGF的表达的影响  

Eeffects of ginkgolides and bilobalide on VEGF expression in rats with focal cerebral ischemia-reperfusion

作　　者：刘秀萍[1] 邓方[1] 

机构地区：[1]吉林大学白求恩第一医院神经内科,吉林长春130021

出　　处：《中风与神经疾病杂志》2015年第2期108-111,共4页Journal of Apoplexy and Nervous Diseases

基　　金：国家自然科学基金(No.81000490;81271293)

摘　　要：目的研究银杏内酯(ginkgolides,GK)和白果内酯(bilobalide,BB)对大鼠脑缺血再灌注损伤后血管内皮生长因子(vascular endothelial growth factor,VEGF)表达的影响,探讨GK和BB能否通过影响血管生成实现神经保护作用。方法制备Wistar大鼠大脑中动脉缺血(middle cerebral artery occlusion,MCAO)模型,缺血2 h后再灌注12 h。将实验动物随机分为4组:假手术组(Sham组)、MCAO+盐水对照组(Saline组)、MCAO+白果内酯组(10 mg/kg,BB组)和MCAO+银杏内酯组(10 mg/kg,GK组)。缺血2 h再灌注12 h后,对大鼠神经功能缺损评分。通过TTC染色观察脑梗死体积变化,应用免疫组化及Western blot检测各组大鼠缺血侧脑皮质区VEGF及其受体VEGFR-1的表达。结果与Saline组相比,GK组和BB组动物神经行为学评分明显降低(P<0.05),脑梗死体积明显减小(P<0.05);与Saline组相比,GK组和BB组VEGF阳性细胞表达数目及蛋白表达均明显增高(P<0.05),而VEGFR-1阳性细胞数表达数目增多,其蛋白表达量未见明显变化。结论银杏内酯和白果内酯对脑缺血/再灌注损伤的保护作用可能与促进VEGF的表达有关。Objective To observe the effects of ginkgolides and bilobalide on the expression of VEGF after cerebral ischemia-reperfusion injury in rats. Methods MCAO（ middle cerebral artery occlusion） models were set up in Wistar rats,after ischemia,for 2 h then reperfusion for 12 h were performed. The animals were divided into four groups： Sham operation group（ Sham group）,MCAO ＋ Saline（ Saline group）,MCAO ＋ ginkgolides group（ GK group） and MCAO ＋ bilobalide group（ BB group）. After ischemia for 2 h then reperfusion for 12 h,the animals＇ neurological deficits and infarct volumes were evaluated. The expression of VEGF and VEGFR-1 were analyzed with immunohistochemical methods and Western blot. Results Compared with Saline group,GK group and BB group showed a significant reduction in infarct volume and neurological deficits（ P 0. 05）. Compared with saline group,the expression of VEGF in brain tissues was increased by immunohistochemical methods and Western blot in GK group and BB group（ P 0. 05）. While the VEGFR-1 expression was increased by immunohistochemical methods but with no change through Western blot assay. Conclusion The findings demonstrate that GK and BB can promote VEGF synthesis relieve the brain damage of cerebral ischemia-reperfusion injury.

关 键 词：银杏内酯 白果内酯 脑缺血再灌注 血管内皮生长因子 

分 类 号：R743.3[医药卫生—神经病学与精神病学]