Foxp3和HO-1在实验性自身免疫性脑脊髓炎大鼠脊髓中的相关性表达及依达拉奉保护作用的研究  

Relationship between expression of Foxp3 and HO-1 in EAE and the protective function of Edaravone

作　　者：李世平[1] 刘露[1] 张美月[1] 王彬[1] 刘瑞春[1] 郭力[1] 董梅[1] 

机构地区：[1]河北医科大学第二医院神经内科,神经病学河北省重点实验室,河北石家庄050000

出　　处：《中风与神经疾病杂志》2015年第2期144-147,共4页Journal of Apoplexy and Nervous Diseases

基　　金：河北省卫生厅项目(No.20130191)

摘　　要：目的探讨叉头样转录因子-3(Foxp3)和血红素加氧酶(HO-1)在实验性自身免疫性脑脊髓炎(EAE)发病中的相关性及依达拉奉对EAE的保护机制。方法建立大鼠EAE动物模型,观察依达拉奉干预前后动物发病情况,应用Real-time PCR方法检测脊髓组织中Foxp3 mRNA和HO-1 mRNA的含量变化;应用免疫组化染色观察脊髓组织Foxp3和HO-1阳性表达。结果依达拉奉能降低EAE的发病率,延迟发病,改善神经功能评分,减少脊髓中炎性细胞浸润和"血管袖套"的形成,促进脊髓中Foxp3和HO-1的转录。结论依达拉奉对EAE和MS具有保护作用,这种作用在病程早期更明显,是通过促进HO-1的表达上调和CD4+CD25+Foxp3+T调节型淋巴细胞(CD4+CD25+Foxp3+Tregs)的发育和功能实现的,具有抗炎和抗氧化的双重作用。Objective To investigate the association between Foxp3 and HO-1 in the course of EAE and the protective effect of Edaravone. Methods Establishing the EAE model and observing the rats incidence before and after the use of Edaravone. The effect of Edaravone was evaluated by comparing morbidity of rats and histopathology of lumbar spinal cords.Furthermore,the use of Edaravone on transcription of Foxp3 and HO-1 in lumbar spinal cords was evaluated by Real-time RCR. Results We found that Edaravone could decrease the incidence rate of EAE,delay the time of attack,alleviate the clinical severity of EAE,reduce the inflammatory cells and＂blood vessel muffs＂in spinal cords,and promote the transcription of Foxp3 and HO-1 in both thymuses and spinal cords. Conclusion Edaravone has protective effect on EAE and MS by upregulating HO-1 and promoting the development and function of CD4＋CD25＋Foxp3＋Tregs. It has the functions of anti-oxidative stress and anti-inflammatory through acting on HO-1.

关 键 词：多发性硬化 实验性自身免疫性脑脊髓炎 FOXP3 HO-1 依达拉奉 

分 类 号：R744[医药卫生—神经病学与精神病学]