束花石斛降血糖活性及化学成分研究  

Study on hypoglycemic activity and chemical constituents from Dendrobium chrysanthum Lindl.

作　　者：倪俊[1] 蔡金艳[1] 黄楚君[1] 吕华冲[1] 陈天洪[1] 

机构地区：[1]广东药学院药科学院,广东广州510006

出　　处：《广东药学院学报》2015年第1期10-13,共4页Academic Journal of Guangdong College of Pharmacy

基　　金：2014年广东省大学生创新创业训练计划项目;2014年学生课外学术科技项目;国家自然科学基金项目(81001628);广东省自然科学基金项目(S2013010014771);广州市珠江科技新星专项

摘　　要：目的研究兰科石斛属植物束花石斛Dendrobium chrysanthumLindl.各部位的降血糖活性及活性部位的化学成分。方法采用胰岛素抵抗Hep G2细胞模型筛选束花石斛降血糖活性部位,并应用多种柱色谱及重结晶的方法对活性部位进行分离纯化,根据化合物的理化性质和波谱数据鉴定化合物结构。结果加药质量浓度为2.0 mg/mL时,三氯甲烷部位的胰岛素抵抗Hep G2细胞葡萄糖消耗量达到了模型组的442.4%,细胞存活数为模型组的62.9%。从三氯甲烷部位分离并鉴定了10个化合物,分别鉴定为β-谷甾醇（1）,moscatin（2）,对羟基反式肉桂酸三十烷基酯（3）,1,3,5-三甲氧基苯（4）,isonuatigentin（5）,香豆素（6）,2,4,7-三羟基-5-甲氧基-9-芴酮（7）,大黄素甲醚（8）,丁香酸（9）,胡萝卜苷（10）。结论综合考虑降血糖活性及对细胞的毒性,束花石斛三氯甲烷部位活性优于其他部位,其中化合物2～6、9为首次从该植物中分离得到,化合物4、5为首次从该属植物中分离得到。Objective To study the hypoglycemic activity of Dendrobium chrysanthumLindl. and chemical constituents from the active part. Methods Insulin resistant Hep G2 cells were used to evaluate the hypoglycemic activity and several chromatographic methods were applied to isolate and purify compounds.The structures were identified on the basis of physicochemical properties and spectroscopic data. Results The glucose consumption and survival cells of 2.0 mg / mL of chloroform extract were 442.4% and 62.9% to model group. Ten compounds were isolated and identified as β-sitosterol（ 1）,moscatin（ 2）,defusine（ 3）,1,3,5-trimethoxybenzene（ 4）,isonuatigentin（ 5）,coumarin（ 6）,2,4,7-trihydroxy-5-methoxy-9-fluorenone（ 7）,physcion（ 8）,syringic acid（ 9）,daucosterol（ 10）. Conclusion The chloroform extract showed significant hypoglycemic activity in Hep G2 cells. Compounds 2 ～ 6 and 9 were isolated from Dendrobium chrysanthumLindl. for the first time,and compounds 4 and 5 were isolated from the genus for the first time.

关 键 词：束花石斛 降血糖活性 化学成分 HEP G2 

分 类 号：R284.2[医药卫生—中药学]