miRNA-191对人乳腺癌细胞增殖、侵袭的影响  

Effect of miR-191 on the proliferation and invasion of human breast cancer cells

作　　者：孙国栋[1] 郑名华 田南南[2,3] 杨建新[4] 

机构地区：[1]广东药学院第一附属医院肿瘤内科,广东广州510080 [2]广东药学院第一附属医院 [3]南方医科大学附属中西医结合医院肿瘤科,广东广州510062 [4]广东药学院第一附属医院乳腺外科,广东广州510080

出　　处：《广东药学院学报》2015年第1期99-103,共5页Academic Journal of Guangdong College of Pharmacy

摘　　要：目的探讨miR-191在人乳腺癌细胞中的表达情况,研究其对乳腺癌细胞的增殖、侵袭的影响。方法 real-time PCR检测乳腺癌细胞[MDA-MB-231(雌激素受体阴性,ER-)、MCF-7(雌激素受体阳性,ER+)]和正常乳腺上皮细胞(HBL-100)中miR-191表达水平的差异;利用Lipofectamine2000将miR-191抑制体瞬时转染乳腺癌MCF-7,并用real-time PCR检测转染效果;分别用CCK-8法检测MCF-7细胞增殖,流式细胞术检测MCF-7细胞的细胞周期,Transwell法检测MCF-7细胞的侵袭能力。结果与正常乳腺细胞相比,miR-191在乳腺癌细胞中高表达(P<0.01),且在ER(+)乳腺癌细胞MCF-7中的表达水平高于ER(-)乳腺癌细胞MDA-MB-231(P<0.01),转染miR-191抑制体后,MCF-7细胞增殖能力(0.65±0.04)较阴性对照组(1.18±0.05)明显受到抑制(F=122.238,P<0.01),侵袭能力(56.67±2.58)较阴性对照组(82.5±5.79)减弱(F=18.734,P<0.01),G0/G1期的细胞比例(73.39±1.10)%较阴性对照组(69.28±2.11)%增加(F=61.060,P<0.01),S期细胞比例(20.9±1.17)%较阴性对照组(25.48±1.19)%减少(F=46.937,P<0.01)。结论 miR-191在人乳腺癌细胞中,特别是ER(+)乳腺癌中高表达,转染miR-191抑制体后,MCF-7细胞增殖、侵袭能力明显受到抑制。Objective To investigate the expression of miR-191 in breast cancer and its effect on the proliferation and invasion of MCF-7cells. Methods The expression of miR-191 in breast cancer cells（ MCF-7 and MDA-MB-231） and normal breast epithelial cell（ HBL^-100） was tested by real-time PCR.Then the miR-191 inhibitor was transfected into MCF-7 cells by lipofectamine2000 and the transfection efficiency was detected by real-time PCR. Cell proliferation was evaluated by CCK-8 assay. The cell cycle was detected by flow cytometry. The cell invasion ability was detected by transwell assay. Results The expression of miR-191 in cancer MCF-7 and MDA-MB-231 cells was significantly higher than that in normal breast epithelial HBL-100 cells（ P〈0.01）. The level of miR-191 in ER positive MCF7 cells was higher than that in ER negative MBA-MD-231 cells（ P〈0. 01）. After transfected with miR-191,the proliferation ability of MCF-7 cells was significantly suppressed,the percentages of cells in G0 / G1 phase increased and the invasion ability of MCF-7 cells was markedly inhibited（ P〈0.01）. Conclusion miR-191 is over-expressed in breast cancer cells,especially in ER positive breast cancer MCF-7 cells. The proliferation and invasion of MCF-7 cells may be suppressed by miR-191 inhibitor.

关 键 词：乳腺癌 MCF-7 MIRNA miR-191 增殖 侵袭 

分 类 号：R737.9[医药卫生—肿瘤]