加味香砂六君子汤对胃癌前病变大鼠胃黏膜细胞凋亡及EGFR,VEGF,C-erb-2蛋白表达的影响  

Modified Xiangsha Liujunzi Apoptosis of Precancerous Gastric Lesions in Rats and EGFR,VEGF,C-erb-2 Protein Expression

作　　者：李康[1] 

机构地区：[1]辽宁省肿瘤医院,辽宁沈阳110042

出　　处：《中华中医药学刊》2015年第3期718-720,共3页Chinese Archives of Traditional Chinese Medicine

摘　　要：目的:观察加味香砂六君子汤对胃癌前病变(PLGC)模型大鼠胃黏膜Bax、Bcl-2、P53mRNA表达的影响,从细胞凋亡角度,探讨该方干预PLGC的基本作用机制。方法:120只健康Wistar大鼠随机分为空白对照组和模型组、维霉素组、加味香砂六君子汤高、中、低剂量组,每组20只,造模24周后开始给予药物干预,给药6周,其后采取免疫组化法检测大鼠胃黏膜中Bcl-2、Bax及P53mRNA表达水平。结果:加味香砂六君子汤各剂量组体质量则持续上升(P﹤0.05),明显高于模型组(76.6±5.2)及维霉素组(86.2±5.1),差异具有统计学意义(P<0.05)。加味香砂六君子汤各剂量组大鼠胃黏膜细胞凋亡指数较模型对照组及维霉素组升高(P<0.05);加味香砂六君子汤高、中、低剂量组间比较,中剂量组最高,但差异无统计学意义(P>0.05)。空白对照组癌前病变大鼠胃黏膜EGFR、VEGF表达最低,模型组表达最高(P<0.05)。加味香砂六君子汤各剂量组癌前病变大鼠胃黏膜EGFR、VEGF表达与模型对照组及维霉素组比较,显著降低,差异明显(P<0.05)。加味香砂六君子汤高、中、低剂量组间比较方面,三剂量组EGFR表达差异不显著,无统计学意义(P>0.05)。高、中剂量组VEGF的表达,与低剂量组相比,明显降低,差异具有统计学意义(P<0.05)。结论:加味香砂六君子汤可促进胃癌前期病变大鼠胃黏膜细胞凋亡;并下调EGFR,VEGF,C-erb-2的表达水平,达到治疗胃癌前期病变的临床疗效。Objective： To observe the Modified Xiangsha Liujunzi of precancerous lesions（ PLGC） rat model of gastric Bax,Bcl- 2,the impact P53 mRNA expression,from the perspective of apoptosis,explore the side interventions PLGC basic mechanism of action. Methods： 120 healthy Wistar rats were randomly divided into control group and model group,Victoria ADM group,flavored Xiangsha Liujunzi high,medium and low dose groups 20,24 weeks after modeling start giving drugs intervention,administered six weeks,followed by immunohistochemical method adopted rat gastric mucosa in Bcl-2,Bax and P53 mRNA expression levels. Results： Modified Xiangsha Liujunzi each dose group weight continued to rise（ P〈 0. 05）,significantly higher than the model group（ 76. 6 ± 5. 2） and Victoria ADM group（ 86. 2 ± 5. 1）,the difference was statistically significant（ P〈 0. 05）. Modified Xiangsha Liujunzi gastric mucosal cells of rats in each dose group compared with the model group apoptosis index and Victoria ADM group increased（ P〈 0. 05）; flavored Xiangsha Liujunzi high,between the low dose group,the highest dose group,but the difference was not statistically significant（ P〈 0. 05）.The control group of rats gastric precancerous lesions EGFR,VEGF expression lowest,highest expression in model group（ P〈 0. 05）. Modified Xiangsha Liujunzi each dose group precancerous lesions in rat gastric mucosa EGFR,VEGF expression with the model group and Victoria ADM group,was significantly lower,the difference was significantly（ P〈 0. 05）.Modified Xiangsha Liujunzi high,medium and low dose group comparisons among the three dose groups was not significantly different EGFR expression,no statistically significant（ P〈 0. 05）. High dose groups of VEGF expression,compared with the low- dose group was significantly lower,the difference was statistically significant（ P〈 0. 05）. Conclusion：Modified Xiangsha Liujunzi precancerous lesions can promote apoptosis in rat gastric mucosa; and reduced EGFR,VEGF,the expr

关 键 词：加味香砂六君子汤 大鼠 胃癌前病变 EGFR VEGF C-ERB-2 

分 类 号：R285.5[医药卫生—中药学]