大鼠Langendorff离体心脏灌流模型的制备经验及其影响因素分析  

Preparation experience on model of Langendorff isolated heart perfusion in rat and analysis of influence factors

作　　者：王小晓[1] 王幼平[1] 余海滨[1] 刘卫红[1] 谢世阳[1] 高原[1] 邢作英[1] 

机构地区：[1]河南中医学院第一附属医院中心实验室,郑州450000

出　　处：《中药药理与临床》2014年第6期184-186,共3页Pharmacology and Clinics of Chinese Materia Medica

基　　金：河南省教育厅科学技术研究重点项目(编号:12A310002)

摘　　要：目的:建立稳定有效的大鼠Langendorff离体心脏灌流模型。方法:采用成年SD大鼠腹腔注射麻醉后,开胸取心,使用改良K-H液恒温、恒流灌注,通过传感器、放大器、信号记录仪,监测记录心室压、灌注压、心脏表面心电图和心脏表面温度。结果:制备成功的大鼠Langendorff离体心脏灌流模型,心室内压保持在75mm Hg左右,心率保持在250次/min左右,节律稳定,无或偶尔发生心律失常,10ml/min恒流灌注,灌注压保持在65mm Hg左右,在持续含氧灌流的情况下,心脏自主搏动可稳定保持2小时以上。结论:严格控制实验过程中的各个环节,熟练掌握操作技术,大鼠Langendorff离体心脏灌流模型均可制备成功,并保持稳定。Objective： Establish stable and effective model of Langendorff isolated heart perfusion in rat. Methods ： Adopting healthy and adult SD rat, after narcosis by intraperition injection, opened chest and gathered heart. Using modifier K-H solution, perfusing with constant temperature and constant flow, monitored and recorded ventricular pressure, perfusion pressure, surface electrocardiogram of heart and surface temperature of heart, through sensor, amplifier , recorder. Resulls： For Successful model of Langendorff isolated heart perfusion in rat, ventricular pressure was 75mmHg around, heart rate was 250 times/min around, rhythm was stabilize,without arrhythmia or happened occasionally, perfusion pres- sure was about 65mmHg at constant flow of 10ml/min, and the heart cound keep beatting rhythmically more than 2h. Conclusion： Through strictly controlling the each lick of experiment and skillfully mastering operation technique, models of Langendorff isolated heart perfusion in rat can be maken successfully and keep stable.

关 键 词：Langendorff离体心脏灌流 模型制备 影响因素 

分 类 号：R-332[医药卫生]