Mechanism of exogenous selenium alleviates cadmium induced toxicity in Bechmeria nivea(L.) Gaud(Ramie)  

外源硒对苎麻的镉胁迫毒性的缓解机制(英文)

作　　者：王春林[1,2] 刘云国[1,2] 曾光明[1,2] 胡新将[1,2] 尹怡诚[1,2] 胡熙[1,2] 周璐[1,2] 王亚琴[1,2] 李华英[1,2] 

机构地区：[1]湖南大学环境科学与工程学院,长沙410082 [2]湖南大学环境生物与控制教育部重点实验室,长沙410082

出　　处：《Transactions of Nonferrous Metals Society of China》2014年第12期3964-3970,共7页中国有色金属学报（英文版）

基　　金：Project(41271332)supported by the National Natural Science Foundation of China;Project(11JJ2031)supported by the Natural Science Foundation of Hunan Province,China

摘　　要：The protective role of exogenously supplied selenium （Se^6＋） on Bechmeria nivea （L.） Gaud （Ramie） subjected to cadmium （Cd） stress was studied in vitro, and the mechanism was discussed by investigating plant growth, malondialdehyde （MDA）, activity of antioxidative enzymes and DNA methylation pattern. Plants grown in hydroponic culture were supplied with spraying Se （selenate, 1.2μmol/L） and cadmium （Cd（NO3）2, 0, 3, 6 and 9 mg/L）, individually or simultaneously. At low Se spraying levels, SOD activity was increased by 35.34%, 43.18%, 3.63%under 3, 6 or 9 mg/L cadmium contents, POD was increased by 12.45%, 14.14%, 3.27%, and the level of DNA methylation was decreased by 10.70%, 18.18%and 15.59%, respectively. The results confirmed that spraying low Se on ramie leaves could enhance the activity of SOD and POD, and regulate DNA methylation in ramie leaves.研究施加外源硒(Se6+)对苎麻镉胁迫毒性的保护机制。主要讨论硒对苎麻生长、MDA、抗氧化酶活性以及DNA甲基化水平的影响。苎麻分别在含镉浓度为0、3、6、9 mg/L条件下培养,并对苎麻叶面喷洒硒酸钠(1.2μmol/L)。结果表明,镉浓度为3、6、9 mg/L营养液中培养的苎麻,经过向叶片喷洒低浓度的硒酸钠(1.2μmol/L)处理后,苎麻叶片中的SOD活性增加了35.34%、43.18%、3.63%,POD活性增加了12.45%、14.14%、3.27%,DNA甲基化下降了10.70%、18.18%、15.59%。结果证实了低浓度硒能强化苎麻SOD、POD活性以及平衡苎麻体内DNA甲基化。

关 键 词：RAMIE SE Cd DNA methylation antioxidative enzyme PHYTOREMEDIATION 

分 类 号：S563.1[农业科学—作物学] Q945.78[生物学—植物学]