亲环素A在人根尖周炎中的表达与临床表现的相关性研究  

Immunolocalization of cyclophilin-A in human apical periodontitis lesions

作　　者：王艳青[1] 胡菊花[2] 李颂[2] 胡淑丽[2] 张红艳[1] 

机构地区：[1]安徽医科大学口腔医学院,安徽合肥230032 [2]安徽医科大学附属口腔医院,安徽省口腔疾病研究中心实验室,安徽合肥230032

出　　处：《牙体牙髓牙周病学杂志》2015年第2期73-78,99,共7页Chinese Journal of Conservative Dentistry

基　　金：安徽省自然科学基金(070413141);安徽高校省级自然科学研究项目(KJ2011Z168);安徽省高校教学质量与教学改革工程项目(20100352)

摘　　要：目的:初步探讨人根尖周炎中亲环素A(Cyp A)表达及与临床表现的关系。方法:收集根尖手术切除并经病理诊断证实的根尖周炎病损组织83例作为实验组;收集新鲜拔除的健康正畸牙牙髓6例作为正常对照组。用免疫组织化学染色法分析比较各组织中Cyp A及RANKL的表达情况,取蛋白平均吸光度值(A值)进行统计学分析,并比较其表达量与临床症状的相关性。结果:根尖周炎病变组织中Cyp A主要表达于炎细胞浸润区和上皮组织,主要阳性细胞为淋巴样细胞、上皮细胞、血管内皮细胞,正常对照组中少见Cyp A的表达;Cyp A在根尖肉芽肿组织中的表达量明显高于根尖囊肿组(P<0.05),RANKL在根尖囊肿组织中的表达量明显高于根尖肉芽肿组(P<0.05);有明显临床症状的根尖周炎组织中Cyp A和RANKL的A值均明显高于无症状组(P<0.05);Cyp A和RANKL的表达水平与根尖周炎病变大小均呈正相关(r=0.462,P<0.05;r=0.417,P<0.05)。结论:Cyp A存在于人根尖周炎病损组织中,可能参与人根尖周炎疾病的发病进程。AIM： To examine the expression of cyclophilin A（ Cyp A） in human periapical lesions and to investigate its relationship with the clinical symptoms of periapical periodontitis. METHODS： Samples of human apical periodontitis lesions of 83 cases were subjected to immunohistochemical examination for Cyp A and RANKL expression. Six 6 healthy dental pulp samples obtained from orthodontic patients served as the controls. The integrated average optical density value（ A） of Cyp A and RANKL was measured by immunohistochemical analysis for all the samples.Differences in Cyp A and RANKL expression between different clinical presentations were subsequently analyzed by t-test. RESULTS： Cyp A postitive cells and RANKL positive cells were both detected in all periapical lesion tissues,and the expression of Cyp A was significantly higher in periapical lesions than in normal tissues（ P〈0. 05）. Epithelial cells,lymphoid cells and endothelial-like cells showed Cyp A positive in the lesions. The expression of RANKL in cyst group were higher than in granuloma group（ Z = 4. 48,P〈0. 05）,while the expression of Cyp A in granuloma group were higher than in cyst group（ Z = 4. 48,P〈0. 05）. The average（ A） value of Cyp A positive sites in periapical lesions with clinical symptoms was higher than that without clinical symptoms（ 77. 24 versus 39. 18,P〈0. 05）. Positive correlation was observed between Cyp A expression and lesion size（ r = 0. 462,P〈0. 05）. Moreover,positive correlation between RANKL expression and lesion size was observed（ r = 0. 417,P〈0. 05）. CONCLUSION： Cyp A might be involved in the inflammatory response and bone resorption,and associated with periapical lesion pathogenesis.

关 键 词：亲环素A RANKL 根尖周炎 临床表现 

分 类 号：R780.2[医药卫生—口腔医学]