Garcinol对人口腔鳞癌细胞的抑制作用研究  

Inhibitory effects of Garcinol on oral squamous cell carcinoma cells

作　　者：周新莹[1] 张辛燕[1] 宿颖[1] 桑圣民 

机构地区：[1]首都医科大学口腔医学院研究所,北京100050 [2]美国北卡农工大学

出　　处：《北京口腔医学》2015年第1期10-14,共5页Beijing Journal of Stomatology

基　　金：国家自然科学基金(81272982);北京市卫生系统高层次卫生人才(2011-3-073)

摘　　要：目的观察Garcinol对人口腔鳞癌细胞系SCC15增殖、细胞周期、凋亡和克隆形成能力的作用。方法培养人口腔鳞癌细胞系SCC15,采用不同浓度的Garcinol处理细胞后,通过MTT法检测对细胞增殖的影响,PI单染色法流式细胞仪检测对细胞周期的影响,Annexin V-FITC/PI双染色法流式细胞仪检测对细胞凋亡的影响,克隆形成实验检测对细胞克隆形成能力的影响。结果 Garcinol能显著抑制SCC15细胞增殖(P<0.01),并呈浓度和时间依赖性。Garcinol能抑制SCC15细胞周期由G1期向S期转变(P<0.01),并呈浓度依赖性。Garcinol还能够诱导SCC15细胞凋亡(P<0.01),并呈浓度依赖性。同时,Garcinol能抑制SCC15细胞的克隆形成能力(P<0.01),并呈浓度依赖性。结论 Garcinol作为一种化学预防制剂,对人口腔鳞癌细胞SCC15具有显著的抑制作用,其机制主要包括抑制SCC15细胞增殖、细胞周期和克隆形成能力,并诱导细胞凋亡。Objective To investigate the effects of Garcinol on proliferation,cell cycle,apoptosis and colony forming ability in oral squamous cell carcinoma（ OSCC） cell line SCC15. Methods OSCC cell line SCC15 were cultured in DMEM： F12 medium with 10% FBS and treated with different concentrations of Garcinol. The cells proliferation was measured by MTT assay. Cell cycle was examined by flow cytometer with PI staining. Cells apoptosis was observed by flow cytometer with Annexin V-FITC / PI staining. Colony formation was examined by clonogenic assay. Results Garcinol could significantly inhibit the proliferation and cell cycle G1 / S phase transition of SCC15 cells in a dose-dependent and timedependent manner（ P〈0. 01）. Garcinol also induced apoptosis in SCC15 cells（ P〈0. 01）. Garcinol could inhibit the colony forming ability of SCC15 cells in a dose-dependent manner（ P〈0. 01）. Conclusion As a chemopreventive agent,Garcinol could significantly inhibit OSCC cell line SCC15. The possible mechanisms include the inhibitory effects of proliferation,cell cycle and colony forming ability in SCC15 cells and its apoptosis-inducing effect.

关 键 词：GARCINOL 口腔鳞癌 

分 类 号：R739.8[医药卫生—肿瘤]