纯钛钛片表面胶原/透明质酸聚电解质复合膜涂层的构建  

Construction of collagen / hyaluronic acid polyelectrolyte multilayer films on the surface of pure titanium disc

作　　者：张锋[1] 韩莉[1] 徐媛媛[1] 任灵飞[1] 蒋颂瑶[1] 施更生[1] 

机构地区：[1]浙江省台州医院口腔科,浙江临海317000

出　　处：《口腔医学》2015年第2期96-100,共5页Stomatology

基　　金：浙江省科技厅公益项目(2014C33280)

摘　　要：目的尝试在纯钛钛片表面构建胶原/透明质酸聚电解质复合膜涂层,并评价其生物相容性。方法纯钛钛片表面经打磨抛光及氧化性混酸溶液处理,得到表面富含钛羟基的基底面(设为对照组),在此基础上接着通过层层自组装技术将I型胶原和透明质酸引入到钛片表面(设为实验组)。采用扫描电镜(SEM)、X射线光电子能谱(XPS)和接触角实验对两组钛片的表面进行表征。最后,在两组钛片表面培养成骨前体细胞,检测其早期粘附、增殖及分化的能力。结果 SEM结果显示,对照组钛片的表面非常平整,高倍镜下发现其表面有很多直径为数十纳米的颗粒状结构。而实验组钛片的表面也很光滑,高倍镜下也可见很多纳米颗粒,但其直径较对照组为小。XPS分析发现实验组钛片表面元素成分氮的含量随着组装层数的增加而不断升高。接触角实验显示实验组钛片表面的可湿性随着钛片表面聚电解质组装层数的变化而不断变化,实验组钛片表面的接触角较对照组小。实验组钛片明显促进了成骨细胞的早期粘附(P<0.05),而且培养在实验组钛片表面的细胞增殖得更快(P<0.05)。同时,在实验组钛片表面生长的成骨细胞分化能力更强。结论 I型胶原和透明质酸能够被成功的组装到纯钛钛片表面,该涂层结构有利于成骨前体细胞的生长,显示了良好的生物相容性。Objective To construct collagen/hyaluronic acid polyeletrolyte multilayer films on the surface of pure titanium disc and to evaluate its biocompatibility. Methods Pure titanium disc was first ground, polished and acid-etched with a mixture of HESO_4/ H_2O_2, and then a basal surface with plenty of hydroxy group was obtained（ set as control group）. Collagen type I and hyaluronic acid was introduced onto this basal surface using layer by layer technique （ set as test group）. Scanning electron microscope （ SEM ） , X-ray photoelectron spectroscopy（XPS） and contact angle measurement were used for surface characterization. Pre-osteoblasts were seeded on these 2 groups＇ discs,and then cell attachment,cell proliferation and cell differentiation were evaluated. Results SEM analysis demon- strated that the pure titanium discs in the 2 groups both had very smooth surfaces,and were also covered with different granules in nano size. However,the nanoparticle of the control group was much bigger than that of the test group. XPS analysis showed that the element nitrogen content of the test surface dramatically increased with deposition number. Contact angle measurement showed that surface wetta- bility of the test surface was dramatically altered with the deposition number. Nevertheless,the contact angle of the test surface was less than that of the control surface. Cell attachment and cell proliferation were much stimulated in the test group. Meanwhile, the cells grew on the surface of test group showed much stronger differentiation ability. Conclusions Collagen type I and hyaluronic acid was suc- cessfully constructed on the titanium surface,and this coating promoted cell growth,showing good biocompatibility.

关 键 词：I型胶原 透明质酸 层层自组装 

分 类 号：R783.1[医药卫生—口腔医学]