血清高尔基体蛋白73联合甲胎蛋白检测在原发性肝癌患者中的诊断价值  

Combined detection of alpha-fetoprotein and Golgi protein 73 for primary hepatic carcinoma diagnosis

作　　者：崔冉亮[1] 李悦国[1] 张鹏宇[1] 张鹏[1] 

机构地区：[1]天津医科大学肿瘤医院检验科,国家肿瘤临床医学研究中心,天津市肿瘤防治重点实验室,国家"863"计划临床研究实验室,天津市300060

出　　处：《中国肿瘤临床》2015年第4期222-225,共4页Chinese Journal of Clinical Oncology

摘　　要：目的:探讨高尔基体蛋白73(golgi protein 73,GP73)联合甲胎蛋白检测用于原发性肝癌诊断的临床意义。方法:收集2013年6月至9月天津医科大学肿瘤医院160例血清,其中原发性肝癌患者46例,单纯肝硬化患者30例,其他消化系统肿瘤患者44例,健康者40例。应用酶联免疫吸附实验定量检测血清GP73水平,电化学发光免疫分析检测血清甲胎蛋白(alpha-fetoprotein,AFP)水平。结果:GP73诊断原发性肝癌的敏感性达到73.9%,与本研究中AFP敏感性(56.5%)相比,前者有显著增高,差异有统计学意义(P<0.01)。GP73和AFP联合检测敏感性有显著提高,可以达到86.9%,与单独一种相比敏感性有显著性差异(P<0.01)。结论:GP73有可能作为原发性肝癌诊断的血清标志物之一,而GP73和AFP的联合检测对原发性肝癌的诊断也有较好的应用价值。Objective：The diagnostic sensitivity of alpha-fetoprotein （AFP） in patients with primary liver cancer is poor and un-satisfactory in the clinical diagnosis of patients with primary liver cancer. Recent studies have demonstrated the upregulation of serum primary hepatic carcinoma （PHC） and Golgi glycoprotein 73 （GP73） in patients with PHC. This study aims to discuss the clinical signif-icance of GP73 and AFP in the serum on the diagnosis of primary liver cancer. Methods：The serum concentrations of GP73 and AFP were retrospectively analyzed in 46 hepatocellular carcinoma patients, 30 cases of patients with liver cirrhosis alone, 44 patients with other digestive tumors, and 40 healthy subjects as controls. Quantitative euzymelinked immunosorbent assay and electrochemilumines-cence immunoassay were conducted to determine the GP73 and AFP levels in the serum, respectively. Results：The sensitivity of PHC diagnosis significantly increased with GP73 （73.9%） compared with AFP （56.5%）, and the difference was statistically significant （P〈0.01）. The sensitivity of AFP combined with GP73 significantly improved to 86.9%compared with the sensitivity of a single indicator （P〈0.01）. Conclusion：GP73 may be used as one of the PHC serum markers for diagnosis. The combined detection of GP73 and AFP offers great potential for future application in the diagnosis of PHC.

关 键 词：肿瘤标志物 GP73 甲胎蛋白 肝细胞癌 

分 类 号：R735.7[医药卫生—肿瘤]