人肝癌鸡胚尿囊膜移植瘤模型的建立及血管生成特征的观察  

Establishment and angiogenesis characteristic of human hepatocellular carcinoma transplantation model of chick chorioallantoic membrane

作　　者：吕祥[1,2] 李文[1] 凌丽[2] 徐燕丰[3] 侯风刚[1] 任建琳[1] 李雁[1] 夏英[2] 

机构地区：[1]上海中医药大学附属市中医医院肿瘤科,上海200071 [2]上海中医药大学附属市中医医院国家药物临床试验机构,上海200071 [3]上海中医药大学附属市中医医院药剂科,上海200071

出　　处：《现代肿瘤医学》2015年第5期585-588,共4页Journal of Modern Oncology

基　　金：国家自然科学基金资助项目(编号:81202958;81473624;81473628);国家中医药重点专科建设项目(编号:ZJ0901ZL020);上海市中医肿瘤特色专科建设项目(编号:2008YSZK008);上海市卫生局科研项目(编号:20124Y018);上海市教委创新项目(编号:12YZ057)

摘　　要：目的:研究人肝癌细胞株Bel-7402鸡胚尿囊膜移植瘤模型建立的方法,观察和分析其血管生成的特征。方法:将不同浓度的人肝癌细胞株Bel-7402接种于鸡胚尿囊膜(chick embryo chorioallantoic membrane,CAM),观察影响肝癌鸡胚尿囊膜移植瘤模型瘤体成活的因素、瘤体生长特征和血管生成情况。结果:建立了人肝癌细胞CAM移植瘤模型。移植瘤模型瘤体易于生长,具有较强的血管生成作用。结论:该移植瘤模型易于复制,能动态观察肝癌的血管生成过程,可用于肝癌的生物学行为、药物筛选等领域的研究。Objective：To establish a human liver cancer model on the chick embryo chorioallantoic membrane （CAM）and study its characteristics of angiogenesis. Methods：Human liver cancer cell line Bel - 7402 at different concentrations were inoculated on the CAM. To observe the factors affecting the survival of the transplanted human liv-er cancer,growth characteristics of the transplanting tumor and the characteristics of angiogenesis. Results：The trans-plantation tumor modeI of human liver cancer in CAM was successfully established. It was found that transplantation tumor was easy to grow and it showed strong angiogenesis effects. Conclusion：It is feasible to establish a transplanta-tion tumor model of human liver cancer on CAM. The model can be easily duplicated,which provides a useful animal model for studying the angiogenesis and biological properties of colorectal cancer and can be used in the research of the drug selection for human liver cancer.

关 键 词：肝癌 鸡胚尿囊膜 模型 动物 血管生成 

分 类 号：R73-354[医药卫生—肿瘤] R735.7[医药卫生—临床医学]