氨基葡萄糖对人肝癌HepG2细胞增殖及凋亡的影响  

Effects of glucosamine on proliferation and apoptosis of human Hep G2 cells and its mechanism

作　　者：吴伟刚[1] 刘威龙[1] 诸葛福艳[2] 田圆[1] 刘琢冰 陈心春[1] 周伯平[1] 

机构地区：[1]广东医学院附属深圳市第三人民医院深圳市肝病研究所深圳市感染免疫重点实验室,广东深圳518112 [2]华南师范大学生物光子学研究院,广东广州510631

出　　处：《现代肿瘤医学》2015年第5期601-604,共4页Journal of Modern Oncology

基　　金：国家自然科学基金面上项目(编号:81372227);深圳市科技计划项目(编号:CXZZ20130515163643)

摘　　要：目的:研究氨基葡萄糖对人肝癌Hep G2细胞增殖及凋亡的影响,并初步探讨其分子机制。方法:CCK-8法检测人肝癌Hep G2细胞的增殖情况。流式细胞术检测细胞周期及细胞凋亡。Western blot检测ERK及P-ERK蛋白的表达水平。结果:氨基葡萄糖可以抑制人肝癌Hep G2细胞的增殖,且该作用随着药物浓度的增加而逐渐增强。1.0mmol/L氨基葡萄糖作用72h可以使G0/G1期细胞比例增加,S期细胞比例降低。同时,2.0mmol/L及4.0mmol/L氨基葡萄糖处理72h可以使人肝癌Hep G2细胞发生凋亡。另外,随着药物浓度的增加,人肝癌Hep G2细胞中ERK蛋白的磷酸化水平逐渐降低。结论:氨基葡萄糖可能是通过降低ERK1/2蛋白的磷酸化水平影响细胞周期,从而抑制人肝癌Hep G2细胞的增殖并诱导其发生凋亡。Objective：To study the effects of glucosamine on the proliferation and apoptosis of human HepG2 cells,and its possible molecular mechanism. Methods：The proliferation of human hepatoma HepG2 cells was detected by CCK - 8 method. Cell cycle and apoptosis were detected by flow cytometry. The expression level of ERK and P -ERK proteins were detected by Western blot. Results：Glucosamine can inhibit the proliferation of human hepatoma HepG2 cells,and the inhibition rate enhanced with the increase of drug concentration. At the same time,treatment with glucosamine of 2. 0mmol∕ L and 4. 0mmol∕ L for 72 hours may induce apoptosis of human hepatoma HepG2. In addition,with the increase of drug concentration,and the phosphorylation level of ERK protein of human hepatoma HepG2 cells decreased gradually. Conclusion：Glucosamine may inhibit the proliferation of human hepatoma HepG2 cells and induce its apoptosis through the phosphorylation level of ERK protein and affect the cell cycle of HepG2 cells.

关 键 词：氨基葡萄糖 肝癌细胞 HEPG2 增殖 凋亡 

分 类 号：R735.7[医药卫生—肿瘤]