人高、低转移大细胞肺癌细胞株中黏附分子存在状态的鉴定  

Identification of adhesion molecules existence in the human high and low metastatic large cell lung cancer cell line

作　　者：郑海霞[1] 申东兰[2] 彭安[2] 何艳玲[2] 

机构地区：[1]北京大学深圳医院体检科,广东深圳518036 [2]北京大学深圳医院肿瘤内科,广东深圳518036

出　　处：《现代肿瘤医学》2015年第6期743-746,共4页Journal of Modern Oncology

基　　金：国家自然科学基金资助项目(编号:30070333)

摘　　要：目的:鉴定人高、低转移大细胞肺癌细胞株L9981和NL9980中黏附分子的存在状态。方法:利用半定量RT-PCR技术检测人高、低转移大细胞肺癌细胞株L9981和NL9980中E-cadherin、integrinβ1、integrinβ3基因的mRNA表达;利用流式细胞仪技术检测人高、低转移大细胞肺癌细胞株L9981和NL9980中E-cadherin、integrinβ1、integrinβ3基因的蛋白表达。结果:人高转移大细胞肺癌细胞株L9981中E-cadherin mRNA与蛋白表达水平显著低于低转移大细胞肺癌细胞株NL9980,而integrinβ1和integrinβ3的mRNA与蛋白表达水平高转移L9981细胞株显著高于低转移NL9980细胞株。结论:E-cadherin高表达与肺癌低转移有关,而integrinβ1、integrinβ3高表达与肺癌高转移有关。Objective：To identify the adhesion molecules existence in the human high and low metastatic large cell lung cancer cell line L9981 and NL9980.Methods： Semi-RT-PCR was used to detect the mRNA expression of E-cadherin,integrinβ1 and integrinβ3 between the human high and low metastatic large cell lung cancer cell line;flow cytometry was used to detect the different protein expression of E-cadherin,integrinβ1 and integrinβ3 between the human high and low metastatic large cell lung cancer cell line.Results：The expression of mRNA and protein of E-cadherin of the high metastatic large cell lung cancer cell line was weaker than that of the low metastatic large cell lung cancer cell line.The expression of mRNA and protein of integrinβ1 and integrinβ3 of the high metastatic large cell lung cancer cell line was stronger than that of the low metastatic large cell lung cancer cell line.Conclusion：The high expression of E-cadherin is related to low metastatic lung cancer,the high expression of integrins is related to high metastatic lung cancer.

关 键 词：肺癌细胞株 钙调素 整合素 肿瘤转移 

分 类 号：R734.2[医药卫生—肿瘤]