LOXL2促进乳腺癌MCF-7细胞侵袭研究  

LOXL2 promotes the invasion of human breast cancer cell line MCF-7

作　　者：曹宇勃[1] 于涛[2] 惠林萍[2] 李嵚[2] 

机构地区：[1]中国医科大学附属第四医院肿瘤内科,辽宁沈阳110032 [2]中国医科大学附属第四医院中心实验室,辽宁沈阳110032

出　　处：《现代肿瘤医学》2015年第6期749-751,共3页Journal of Modern Oncology

基　　金：国家自然科学基金(编号:31201053;81372337)

摘　　要：目的:研究赖氨酰氧化酶样蛋白-2(lysyl oxidase like-2 protein,LOXL2)对乳腺癌MCF-7细胞侵袭转移的影响。方法:利用脂质体法将构建好的Flag-LOXL2质粒转染到乳腺癌MCF-7细胞中,应用Western blot检测转染效率,Transwell侵袭实验检测乳腺癌MCF-7细胞的体外侵袭力,并进一步使用Western blot检测LOXL2蛋白对金属蛋白酶MMP2/MMP9表达的影响。结果:在乳腺癌MCF-7细胞中瞬时转染FlagLOXL2,LOXL2蛋白表达明显增加,并促进MCF-7细胞的侵袭能力,上调金属蛋白酶MMP2和MMP9蛋白的表达。结论:LOXL2促进MCF-7细胞的侵袭能力,并影响金属蛋白酶MMP2以及MMP9的表达。Objective：To observe LOXL2 how to affect the invasion of human breast cancer MCF-7 cells.Methods：Flag-LOXL2 plasmid was transfected into human breast cancer MCF-7 cells,the changes of LOXL2 protein expression were assayed by using Western blotting.Transwell invasion assay was used to assess the invasive ability of breast cancer cells.Western blot detected the effect of LOXL2 protein on the expression of MMP2 / MMP9.Results：Flag-LOXL2 plasmid was transiently transfected into human breast cancer MCF-7 cells,the expression of LOXL2 protein increased significantly,which promoted the invasion of MCF-7 cells and upregulated the expression of MMP2 / MMP9.Conclusion：LOXL2 promotes the invasion of human breast cancer MCF-7 cells and raises the expression of MMP2 / MMP9.

关 键 词：赖氨酰氧化酶样蛋白-2 侵袭 乳腺癌 

分 类 号：R737.9[医药卫生—肿瘤]