TSLC1基因对食管癌细胞EC109增殖与侵袭能力抑制的实验研究  

Inhibitory effect of TSLC1 gene on proliferation and invasion ability in esophageal carcinom a cell EC109

作　　者：冉永刚[1] 游颜杰[2] 张晓辉[3] 王海波[4] 郭旭[1] 赵新雷[1] 

机构地区：[1]中国人民解放军白求恩医务士官学校训练部,河北石家庄050081 [2]漯河医学高等专科学校药学系,河南漯河462000 [3]河南科技大学第一附属医院放射科,河南郑州471000 [4]中国人民解放军白求恩医务士官学校生理教研室,河北石家庄050081

出　　处：《现代肿瘤医学》2015年第6期755-759,共5页Journal of Modern Oncology

基　　金：河南省科技攻关计划项目(编号:142102310464);漯河医学高等专科学校自然科学项目(编号:2013;Y-J You)

摘　　要：目的:探讨肺癌抑癌基因1(TSLC1)对食管癌细胞株EC109增殖与侵袭转移能力的影响。方法:采用RT-PCR法获得TSLC1基因全长编码区序列,构建pc DNA3.1-TSLC1真核表达载体并稳定转染食管癌细胞株EC109,以MTT法测定细胞增殖水平,以细胞黏附、侵袭与迁移实验分别检测细胞黏附、侵袭与转移能力。结果:成功构建TSLC1真核表达载体,稳定转染EC109细胞后可显著抑制肿瘤细胞的增殖、黏附、侵袭与迁移能力。结论:过表达TSLC1基因对食管癌细胞的恶性生物学特征显示明显的抑制作用,为进一步研究TSLC1在食管癌发生和进展中的作用奠定了实验基础。Objective：To investigate the inhibitory effects of TSLC1 gene on proliferation and invasion of esophageal carcinoma cell line EC109.Methods：Coding area of TSLC1 gene was obtained by RT-PCR and cloned into eukaryotic expression vector pc DNA3.1（ pc DNA3.1-TSLC1）.Tumor cell activities of proliferation,adherence,invasion and migration were measured by MTT assay,adhesion assay,invasion or migration assay.Results：The eukaryotic expression vector was constructed correctly and stable transfection of pc DNA3.1-TSLC1 significantly suppressed the abilities of proliferation,adhesion,invasion and migration in EC109 cells（ P〈0.05）.Conclusion： Expression of TSLC1 gene has a significant inhibitory effect on the malignant phenotypes in nasopharyngeal carcinoma cell line EC109,provide an experimental basis for investigating effects of TSLC1 in the occurrence and development of esophageal carcinoma.

关 键 词：食管癌 TSLC1 增殖 侵袭 

分 类 号：R735.1[医药卫生—肿瘤]