无刺花椒嫁接愈合过程中相关生理指标的变化  

Changes of Relative Physiological Indices Involved in the Graft Union Development of Zanthoxylum bungeanum

作　　者：朱晓慧[1] 杨途熙[2] 魏安智[2] 冯世静[2] 蒋弘刚 侯娜 刘永红[2] 

机构地区：[1]西北农林科技大学生命科学学院,陕西杨陵712100 [2]西北农林科技大学林学院,陕西杨陵712100 [3]贵州省林业科学院,贵州贵阳550005

出　　处：《西北林学院学报》2015年第2期134-138,共5页Journal of Northwest Forestry University

基　　金：西北农林科技大学项目(TGZX2012-07;XNY2013-10)

摘　　要：以"无刺花椒/凤县大红袍"、"凤县大红袍/凤县大红袍"2种嫁接组合为试验材料,研究了花椒嫁接体愈合过程中POD酶、PPO酶活性及IAA、总酚、可溶性糖含量的变化。结果表明,在愈合过程中,嫁接体接合部的POD酶、PPO酶活性及IAA含量随愈合时间的延长呈"先增加后降低"的变化,其中在嫁接后的第14天增加显著,且达到最大。愈合植株及未愈合植株接合部的总酚含量均随着时间的延长而增加,但未愈合植株的总酚含量显著高于愈合植株。愈合植株接合部的可溶性糖含量随着时间的延长而逐渐减少,而未愈合植株中可溶性糖含量逐渐增加,至嫁接后第22天,未愈合植株中可溶性糖含量显著高于愈合植株。POD酶和IAA含量的增加有利于接合部的愈合,而总酚的累积不利于愈合。嫁接后的2周是花椒愈合的关键时期。In this study,changes of auxin,phenol and soluble sugar content,activities of peroxidase and polyphenol oxidase were monitored in the graft union development of Zanthoxylum bungeanum with Wucihuajiao as scion and Dahongpao as stock.Meanwhile,Dahongpao was self-grafted,which was taken as the control.The results showed that the activity of peroxidase,polyphenol oxidase and auxin increased at first and then decreased.They increased significantly and reach max at 14d.Phenols content in healed and unhealed plants kept increasing,while in unhealed plants it was significantly higher than healed plants.Soluble sugar content in healed plants decreased gradually.However,it kept increasing in the unhealed plants,which became significantly high compared to healed plants at 22d.These results suggested that increased peroxidase and polyphenol oxidase activities,auxin content might be favorable to graft union development in Z.bungeanum,and the accumulation of phenols might be unfavorable to graft union development.The crucial healing time of Z.bungeanumis 2 weeks after grafting.

关 键 词：花椒 接合部 过氧化物酶 多酚氧化酶 生长素 总酚 可溶性糖 

分 类 号：S723.2[农业科学—林木遗传育种]