快原子轰击离子化质谱研究酚类化合物抗氧化作用  

Development of Fast Atom Bombardment Mass Spectrometry for Determination of Antioxidant Activity of Phenolic Compounds

作　　者：杨芬[1,2] 贺玖明[1] 张瑞萍[1] 再帕尔.阿不力孜 

机构地区：[1]中国医学科学院/北京协和医学院药物研究所天然药物活性物质与功能国家重点实验室,北京100050 [2]北京肿瘤医院,北京100142

出　　处：《分析化学》2015年第2期233-238,共6页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(No.30371671)资助项目~~

摘　　要：为了建立简便、快速筛选高效、低毒的新型抗氧化药物的方法,本研究采用快原子轰击离子化质谱(FAB-MS)对酚类化合物抗氧化作用强弱进行了研究。考察了基质、轰击时间和样本浓度对FAB-MS谱中分子离子(M+·)相对强度的影响,研究了黄酮类化合物在FAB-MS谱中的I(M+·)/I([M+H]+)比值与其抗氧化活性的相关性。对12种酚类化合物的抗氧化活性进行了预测与评价,并与硫代巴比妥酸法(TBA)检测的脂质过氧化抑制率进行比较,验证FAB-MS方法的可靠性。结果表明,由FAB-MS获得的I(M+·)/I([M+H]+)比值能较好地反映酚类化合物抗氧化作用的强弱,可以作为抗氧化剂的体外筛选或预测方法,有助于加速新型抗氧化药物的发现。A fast atom bombardment mass spectrometric method to predict and detect the antioxidant ability of phenolic compounds was developed to accelerate the pace of finding the antioxidant with higher effect and low toxicity. The effect of experimental conditions on the relative peak intensity ratio of M＋· ion to [ M＋H]＋ion in the FAB mass spectra of the compound was investigated, including matrix, scan time and concentration. The correlation of antioxidant activity with the I （ M＋· ）/I （ [ M＋H ]＋） value of flavonoids obtained in FAB mass spectra was studied. Then the antioxidant activity of 12 phenolic compounds was predicted using the above method and the results were compared with those obtained from thiobarbituric acid （ TBA） method. The results show that the I（ M＋· ）/I（ [ M＋H]＋） value of the phenolic compound obtained from FAB mass spectra could reflect their antioxidant activity, which could help to accelerate the development of the antioxidant drug.

关 键 词：抗氧化活性 快原子轰击离子化质谱 酚类化合物 黄酮类化合物 

分 类 号：TQ460.72[医药卫生—药物分析学] O657.63[化学工程—制药化工]