奈达铂提高鼻咽癌CNE-2细胞放射敏感性的体外实验研究  

Experimental research of Nedaplatin to enhance radiosensitizing effects in Human Nasopharyngeal Carcinoma Cell Line CNE-2

作　　者：王镇南[1] 唐志[1] 喻嫦娥[1] 李淑慧[1] 杨东红[1] 

机构地区：[1]广东医学院附属医院肿瘤中心,广东湛江524001

出　　处：《吉林医学》2015年第7期1277-1281,共5页Jilin Medical Journal

摘　　要：目的:研究奈达铂(NDP)对鼻咽癌细胞的放射增敏作用及其机制。为寻求治疗鼻咽癌的新策略奠定理论基础。方法:用CCK-8法检测奈达铂对鼻咽癌CNE-2细胞增殖影响和IC50;用克隆形成实验分析奈达铂对CNE-2细胞的放射增敏作用。用流式细胞术(FCM)检测奈达铂对CNE-2细胞凋亡和周期的影响。结果:奈达铂能够抑制CNE-2细胞的增殖,其IC50为32.576 mg/L。1/5IC50浓度(6.515 mg/L)的奈达铂联合放疗与单纯放疗相比,其放射增敏比(SER)为1.542。奈达铂可诱导CNE-2细胞凋亡,奈达铂组、单纯放疗组凋亡率为(22.7+2.24)%、(30.4+2.17)%(P<0.05),奈达铂加放疗组凋亡率最高,为(44.3+1.85)%(P<0.01)。奈达铂作用48 h,对CNE-2细胞周期分布的改变无明显影响。结论:奈达铂通过增强鼻咽癌细胞的凋亡增加放射敏感性。Objective The aim of the present study was to investigate the effects of NDP on radiation sensitization and mechanism of nasopharyngeal carcinoma cell,to investigate the theoretical basis for the new strategy for the treatment of nasopharyngeal carcinoma. Method The effect of NDP on proliferation and IC50 of CNE-2 cells was detected by CCK-8 assay. The effect of NDP on radiation sensibilization of CNE-2 cells was detected by Clone formation test. The effect of NDP on apoptosis and periodic variation of CNE-2 cells were detected by FCM assay. Results NDP could significantly reduce the growth of CNE-2 cells,the IC50 was 32. 576 mg / L. NDP could sensitize the CNE-2cell to radiation with sensitization enhanencement ratio（ SER） of 1. 542. NDP can induce apoptosis in CNE-2 cells. The apoptosis rate of NDP group,and radiotherapy group was（ 22. 7 ＋ 2. 24） % 、（ 30. 4 ＋ 2. 17） %（ P〈 0. 05）,the NDP plus radiation group was（ 44. 3 ＋ 1. 85） %（ P〈 0. 01）. NDP had no effect on periodic variation of nasopharyngeal carcinoma CNE-2 cell lines. Conclusion NDP could sensitize the CNE-2 cell to radiation by induce its apoptosis.

关 键 词：奈达铂 鼻咽癌 放疗增敏 凋亡 

分 类 号：R739.63[医药卫生—肿瘤]