神经病理性疼痛大鼠脊髓IL-33蛋白表达的变化  

EXPRESSIONS OF SPINAL IL-33 IN A RAT MODEL OF NEUROPATHIC PAIN

作　　者：罗剑刚[1,2] 左玲[1] 许维澄[1] 林小雯[1] 李芸[1] 赵菲[1] 苗贵申 孙涛[1] 傅志俭[1] 

机构地区：[1]山东大学附属省立医院疼痛科,济南250021 [2]中南大学湘雅医院麻醉科,长沙410008

出　　处：《中国疼痛医学杂志》2015年第2期88-91,共4页Chinese Journal of Pain Medicine

基　　金：国家自然科学基金资助项目(81271346);山东省自然科学基金资助项目(ZR2010HM097)

摘　　要：目的：观察外周神经慢性压榨性损伤（chronic constrictive injury,CCI）大鼠脊髓白介素-33（interleukin-33,IL-33）表达的变化规律。方法：雄性Wistar大鼠60只,分为手术组和假手术组,每组30只,于CCI前1天、CCI后第1、4、7、14、21天各时间点测定机械痛阈及热痛阈后立即处死大鼠,每个时间点5只,取L4~6脊髓,采用Western blot方法测定IL-33的蛋白表达变化。结果：手术组大鼠术侧机械痛阈及热痛阈明显降低,脊髓IL-33水平表达增加,明显高于对侧和假手术组术侧;IL-33蛋白水平于CCI后第1天开始增加,第7天达到高峰（P〈0.01）,于CCI后第21天仍维持于较高水平（P〈0.05）。结论：CCI致大鼠脊髓IL-33活化,参与神经病理性疼痛的调控过程。Objective： To investigate possible change of interleukin（IL）-33 in the spinal cord of rats with chronic constriction of the sciatic nerve（CCI）. Methods： Sixty male Wistar rats were randomly divided into sham-operation and CCI groups（n = 30 each）. Each group was further divided into 6 subgroups（n = 5 each） in which the animals were sacrificed for further experiments on one day prior to surgery（baseline） and 1, 4, 7, 14, and 21 days after operation. The mechanical and thermal nociceptive thresholds were assessed by measuring paw withdrawal latency（PWL） to the von Frey filaments and radiant heat stimulation, respectively, at different time points. Protein expression of IL-33 in the lumbar segments of the spinal cord（L4~6） was examined by Western blot analysis. Results： The mechanical and thermal nociceptive thresholds decreased significantly in the side ipsilateral to CCI. The protein expression of IL-33 in spinal cord ipsilateral to CCI increased significantly compared with those in groups of contralateral to CCI and sham-operation. The protein expressions of IL-33 began to increase at day 1, peaked at day 7, and maintained at a high level throughout the experiment until day 21. Conclusions：Activation of spinal IL-33 may be involved in the procession of neuropathic pain after CCI.

关 键 词：神经病理性疼痛 慢性坐骨神经缩窄损伤 白介素-33 大鼠 

分 类 号：R741[医药卫生—神经病学与精神病学]