HMGB1和NF-κBp65在葡萄胎、绒癌中的表达变化及意义  

Expression and significance of HMGB1 and NF-κ B in hydatidiform mole and choriocarcinom

作　　者：张涛[1] 刘筠[1] 管英俊[2] 陈燕春[2] 

机构地区：[1]潍坊市中医院产科,山东潍坊261041 [2]潍坊医学院组织学与胚胎学教研室,山东潍坊261053

出　　处：《中国优生与遗传杂志》2015年第3期23-26,F0003,共5页Chinese Journal of Birth Health & Heredity

摘　　要：目的研究早期妊娠、晚期妊娠的胎盘组织、葡萄胎和绒癌组织中高迁移率族蛋白1(HMGB1)和核因子-κBp65(NF-κBp65)的表达变化及意义,探讨HMGB1和NF-κBp65与滋养细胞疾病的关系。方法选择2010年6月至2013年12月潍坊市中医院和潍坊市人民医院收治的流产、剖宫产、葡萄胎和绒癌标本,应用免疫组织化学方法检测HMGB1及NF-κBp65在早期妊娠、晚期妊娠的胎盘组织、葡萄胎和绒癌组织中的表达变化。结果 1.HMGB1阳性颗粒主要定位于细胞质和细胞核中,主要表达于绒毛滋养细胞和血管内皮细胞和间质细胞。与早期和晚期妊娠绒毛组织相比较,葡萄胎和绒癌组织中HMGB1阳性着色强度均明显增强,差异有统计学意义(P<0.001)。2.NF-κBp65阳性颗粒主要定位在细胞质中,主要分布于绒毛滋养细胞、绒毛间质、血管内皮细胞。与早期和晚期妊娠绒毛组织相比较,葡萄胎和绒癌组织中NF-κBp65阳性着色强度均明显增强,差异有统计学意义(P<0.001)。3.HMGB1与NF-κBp65平均光密度值在葡萄胎中表达强度呈正相关(r=0.7499,P=0.000);在绒癌组织中表达强度呈正相关(r=0.7338,P=0.000)。结论 HMGB1及NF-κBp65高表达与葡萄胎、绒癌的发生相关;HMGB1和NF-κBp65在葡萄胎和绒癌组织中的表达趋势一致,两者线性相关分析为正相关,提示HMGB1高表达可增强NF-κBp65的表达,与肿瘤细胞的生长有关。Objective：To explore the change of expression of high mobility group protein（HMGB1）and nuclear factor-κBp65（NF-κBp65）in normal pregnancy,hydatidiform mole and choriocarcinom,further reveal the biological characteristics of the cells of placental site trophoblastic tumor. Methods：Specimen from abortion,cesarean section,hydatidiform mole and choriocarcinoma patients were collected in Weifang Hospital of Traditional Chinese Medicine and Weifang People′s Hospital from 2010 to 2013.The expression of HMGB1 and NF-κBp65 in early pregnancy,the placenta in late pregnancy and hydatidiform mole and choriocarcinoma were detected with immunohistochemical method. Results：1.HMGB1 positive cells were detected in early pregnant,late pregnancy,hydatidiform mole and choriocarcinoma which were localized in the cytoplasm and nucleus. mainly distributes in trophoblasts and vascular endothelial cells and interstitial cells. Compared with the early pregnant and late pregnancy,the intensity of the HMGB1 positive cells is remarkably strengthened in hydatidiform and choriocarcinoma,and there was the statistical significance（P〈0.001）. 2.Immunohistochemistry result showed that the NF-κ B p65 positive granules mainly locate in the cytoplasm,mainly distributes in trophoblasts and vascular endothelial cells and interstitial cells. Compared with the early pregnant and late pregnancy,the intensity of the NF-κ B p65 positive cells is remarkably strengthened in hydatidiform and choriocarcinoma,the difference has the statistical significance（P〈0.001）. 3.The average optical density of the HMGB1 and NF-κ Bp65 in the hydatidiform mole is a positive correlation（r=0.7499,P=0.000）. The HMGB1 and NF-κ Bp65 in choriocarcinoma is a positive correlation（r=0.7338,P=0.000）. Conclusion：1.It suggests that the high expression of the HMGB1 and the NF-κ Bp65 was closely related to hydatidiform and choriocarcinoma. 2.The expression of the HMGB1 and NF-κ Bp65 in hydatidiform and choriocarcinoma have the same trend. Linear

关 键 词：HMGB1 NF-ΚBP65 早期妊娠 晚期妊娠 葡萄胎 绒癌 

分 类 号：R737.33[医药卫生—肿瘤]