ZDF大鼠胃袖状切除附加空回肠侧侧吻合术动物模型的建立  

Establishment and analysis of the rat model of sleeve gastrectomy plus side-to-side jejunoileal anastomosis in ZDF rats

作　　者：王恺京[1] 徐安安[1] 朱江帆[1] 周小钢[1] 高玮[1] 陆佳军 刘晓慧[1] 陈志国[1] 殷晓琦 

机构地区：[1]同济大学附属东方医院,上海200120

出　　处：《腹腔镜外科杂志》2015年第1期1-4,共4页Journal of Laparoscopic Surgery

基　　金：上海市浦东新区卫生局面上项目(编号:pw2013A-3)

摘　　要：目的:为2型糖尿病治疗机制方面的研究提供稳定、持久的降糖手术模型,用肥胖糖尿病(Zucker diabetic fatty,ZDF)大鼠建立胃袖状切除加空回肠侧侧吻合术(sleeve gastrectomy plus side-to-side jejunoileal anastomosis,JI-SG)动物模型。方法:将30只7周龄雄性ZDF大鼠随机分为3组,每组10只,分别为JI-SG组、假手术(sham operation,SO)组(SO组)、饮食控制组(对照组)。观察并测量各组大鼠术前1 d及术后1、3、6周体质量、空腹血糖。结果:手术大鼠存活率为90%(27/30),JI-SG组1只大鼠于术后第1天死亡,2只大鼠于术后3周后死亡。3组大鼠术前体质量、空腹血糖差异无统计学意义。对照组经饮食控制1周后体重上升缓慢,恢复正常饮食后体重迅速上升。JI-SG组及SO组术后大鼠体质量均进行性下降。SO组术后第7天体质量降至最低体重,此后明显上升;JI-SG组术后体重持续下降,术后6周,JI-SG大鼠体重明显小于其他两组,差异有统计学意义(P<0.05)。术后对照组血糖稳步上升,SO组血糖下降后迅速上升,JI-SG组大鼠血糖逐渐下降至正常水平,术后6周3组间血糖差异有统计学意义(P<0.05)。结论:本研究成功建立了ZDF大鼠JI-SG手术模型,此术式明显降低了ZDF大鼠体重、改善了ZDF血糖代谢,为2型糖尿病治疗机制的研究提供了一种稳定、持久的降糖手术模型。Objective： To establish a steady and persistent hypoglycemic model of sleeve gastrectomy plus side-to-side jejunoileal anastomosis（ JI-SG） with Zucker diabetic fatty（ ZDF） rat for the study of therapeutic mechanism of type 2 diabetes mellitus.Methods： Thirty 7-week-old male ZDF rats were randomly divided into three groups： JI-SG group,sham operation（ SO） group and diet control group,every group had 10 rats. Body mass and fasting blood glucose of rats were observed and measured in preoperative 1 d and1,3,6 weeks after surgery. Results： After surgery 90% rats（ 27 /30） survived,in JI-SG group,a rat died at 1 d,and 2 rats died in three weeks after surgery. Three groups of pre-operative rats had no significant differences in body mass and fasting blood glucose. Rats in control group were controlled on diet when other rats accepted surgeries,their weight rose slowly during that time,but weight increased rapidly when they returned to normal diet. JI-SG group and SO group had a progressive weight loss after surgery. Weight of SO group was down to the lowest level on the seventh day after surgery and then increased significantly; Weight of JI-SG group continued to decrease after surgery,body weight of JI-SG rat was significantly smaller than the other two groups in 6 weeks after operation（ P〈0. 05）. After surgery,blood glucose of control group was increased steadily,SO group decreased after surgery and then rose rapidly,JI-SG group gradually decreased to normal level,after 6 weeks the differences among 3 groups were statistically significant（ P〈0. 05）. Conclusions：This study establishes the JI-SG model in ZDF rats,which can significantly reduce the body weight of ZDF rats,and improve glucose metabolism. This procedure provides a stable and persistent operative model for the study of the treatment mechanism of type 2 diabetes.

关 键 词：胃袖状切除术 空回肠侧侧吻合术 肥胖症 大鼠 Zucker 糖尿病 2型 模型 动物 

分 类 号：R-332[医药卫生] R656.61