三维微纳米制造技术在癌症生物物理研究中的应用  

3D micro/nano fabrication and its application in cancer biophysics

作　　者：鲁金蕾 王晓晨[1] 容晓晖[1] 刘雳宇[1] 

机构地区：[1]中国科学院物理研究所,软物质重点实验室,北京100190

出　　处：《物理学报》2015年第5期8-20,共13页Acta Physica Sinica

基　　金：国家重点基础研究发展计划(批准号:2013CB837200);国家自然科学基金(批准号:11474345)资助的课题~~

摘　　要：癌症致命的主要原因是癌细胞在临床上的转移性.癌细胞的侵袭和转移是一个非常复杂的三维过程,但现有的癌症研究在活体上有诸多观测和操作上的困难.而体外实验又通常在培养皿中进行,其二维的生长环境已完全不能满足对癌细胞空间转移性的深入研究,故在活体外构建出癌细胞侵袭和转移的三维物理模型具有十分重要的意义.然而如何在体外尽可能真实地模拟体内癌细胞的生长微环境一直是困扰科学家的难题.本文系统介绍了三维微纳米制造的几种主流技术,探讨了它们在癌症生物物理研究中的应用和发展.在此基础上为了在未来实现对体外三维模型的制造、观测和精确操作,文章还创新性地提出了一种结合紫外线固化生物型水凝胶的三维成型技术、光片三维成像技术以及微纳米探针控制技术的一体化研究平台.这些先进的技术和理念,势必会逐步升级现有传统的癌症研究手段,为未来理解和治疗癌症揭开全新的篇章.The major reason why cancer kills is its metastatic potentials. Metastasis is an extremely complex three-dimensional （3D） process. Currently, routine in vivo cancer research still experiences bottlenecks in observation and manipulation, while in vitro research mainly stays in petri-dish levels that limit cell environment with two-dimensional confinements. Therefore, it is in urgent needs to construct 3D in vitro models to deepen the studies in cancer invasion and metastasis. However“how to carry out” is a big question that harasses scientists for decades. In this report, we will systematically discuss several popular technologies for 3D microfabrication and their applications in bio and cancer research. Besides, we will demonstrate our efforts to construct an all-in-one 3D micro ecology simulation system （3D MESS） which highly integrates a UV bio-compatible hydrogel printer, a light-sheet imaging system, and a nano pipette operation system. This novel system will create a brand-new method to fabricate, observe and controls the 3D structures and the cells inside. We believe that these advanced technology and ambitions will definitely update the current cancer research methods and bring promising hopes for future deeper understandings and more e？cient treatment of cancer.

关 键 词：三维微纳米制造技术 3D生物打印 体内微环境 生物物理 

分 类 号：R73-3[医药卫生—肿瘤]