敲低PES1基因抑制舌癌细胞的生长  

Knockdown of PES1 inhibits tongue squamous cell cancer cell growth

作　　者：任伟[1,2] 程龙[2] 杜佩云[2] 姜丽娜[2] 营孙阳[2] 林佳佳[1] 张菊会[1] 叶棋浓[2] 王恩群[1] 

机构地区：[1]安徽医科大学附属安庆市立医院口腔颌面外科,安徽安庆246003 [2]军事医学科学院生物工程研究所,北京100850

出　　处：《军事医学》2015年第1期40-43,共4页Military Medical Sciences

基　　金：国家自然科学基金资助项目(31200565);北京市自然科学基金资助项目(5132027)

摘　　要：目的构建稳定表达PES1 shRNA的舌癌细胞系,研究敲低PES1基因对舌癌细胞生长的影响。方法在293T细胞中包装并获得敲低PES1基因的病毒,然后将病毒感染Tca8113、SCC6和SCC15 3种舌癌细胞并筛选稳定克隆,Western印迹检测PES1蛋白的表达水平;利用生长曲线检测敲低PES1对舌癌细胞生长的影响,流式细胞术检测敲低PES1对舌癌细胞周期的影响。结果成功构建稳定表达PES1 shRNA的舌癌细胞系,敲低PES1基因能够抑制舌癌细胞的生长,使细胞周期阻滞在G0/G1期,敲低PES1抑制cyclin D1的表达。结论敲低PES1抑制舌癌细胞的生长,诱导细胞周期阻滞在G0/G1期,抑制cyclin D1的表达,因此PES1可能成为舌癌基因治疗的靶标。Objective To construct PES1 shRNA stable expression cell lines in tongue squamous cell carcinoma（TSCC） cells and to study the effect of knockdown of PES1 on the growth of TSCC cells.Methods Recombinant lentivirus carrying PES1 shRNA was packaged and obtained in 293 T cells.TSCC cells（Tca8113,SCC6 and SCC15） were infected with the lentivirus and selected for stable cells.PES1 expression was identified by Western blot.The effect of inhibition of PES1 on the growth and cell cycle of TSCC cells was detected by growth curve and flow cytometry.Results TSCC cells stably expressing PES1 shRNA were constructed.Knockdown of PES1 inhibited cell proliferation and induced cell cycle arrest at G0/ G1 phase.Knockdown of PES1 inhibited expression of cyclin D1 in TSCC cells.Conclusion Inhibition of PES1 results in reduced cell proliferation,cell cycle arrest at G0/ G1 phase and reduction of cyclin D1 expression in TSCC cells.PES1 may be a target for TSCC gene therapy.

关 键 词：PES1 舌肿瘤 细胞生长 细胞周期 细胞周期蛋白D1 

分 类 号：R739.86[医药卫生—肿瘤]