水稻辐射诱变突变体的种子蛋白质成分分析  

Composition Analysis of Seed Protein in Rice Mutants Induced by ^(60)Co γ-Ray

作　　者：曲龙[1] 卢夏茹 李阳生[2] 韦双双[1] 袁潜华[1] 马启林[1] 

机构地区：[1]海南大学热带作物种质资源保护与开发利用教育部重点实验室,海南海口570228 [2]武汉大学/杂交水稻国家重点实验室,湖北武汉430072

出　　处：《核农学报》2015年第2期209-214,共6页Journal of Nuclear Agricultural Sciences

基　　金：国家自然科学基金项目(31160259);转基因作物重大专项(2009ZX08001-020B);海南大学科研基金项目(kyqd1045)

摘　　要：为了改良水稻品种的品质性状,利用60Coγ-射线为诱变源,对粳稻品种Koshihikari种子进行了辐照处理,通过改良的单粒种子法对种子蛋白质的含量和组成进行了SDS-PAGE和Western Blotting分析,筛选到了72个种子蛋白突变体,结果表明经诱变后种子蛋白的含量和组成成分发生了很大变异。本研究为揭示水稻贮藏蛋白积累机制及开发新型稻米种质提供了理论依据。In order to improve the quality traits of rice varieties, a variety ＇ Koshihikari＇ , was radiated by6 0Co γ/-ray to induce mutagenesis. Seventy two mutants of seed storage protein were found through analyzing the content and composition of seed storage protein by SDS-PAGE and western blotting. These mutants reveal rich variations on content and composition of seed protein, and can be used as basis materials to reveal the mechanism of rice storage protein accumulation, and to development new types of rice varieties.

关 键 词：水稻 辐射诱变 种子蛋白 突变体 

分 类 号：S511.22[农业科学—作物学]