响应面优化倍花水解制备没食子酸的工艺条件  

Optimization on Preparation Technological of Gallic Acid From Hydrolysis Chinese Gall Beihua With Response Surface Methodology

作　　者：欧阳玉祝[1] 李雪峰[1] 符智荣[1] 张小凤[1] 

机构地区：[1]吉首大学植物资源保护与利用湖南省高校重点实验室,湖南吉首416000

出　　处：《核农学报》2015年第2期321-326,共6页Journal of Nuclear Agricultural Sciences

基　　金：吉首大学植物资源保护与利用湖南省高校重点实验室开放基金项目(13jdzb094)

摘　　要：为了开发倍花深加工产品,本文以倍花为原料,用浓硫酸催化水解制备没食子酸。在单因素试验基础上,采用响应面优化试验考察了水解温度、水解时间和硫酸用量对没食子酸得率的影响。结果表明,在50m L倍花提取液中,用2.47m L 98%浓硫酸催化,97℃温度下水解315min,没食子酸得率为36.01%,试验结果与试验模型预测值相吻合。分离产物用高效液相色谱结合没食子酸标样进行定性分析,结果与标准品一致。本研究能为倍花的开发利用提供理论依据。In order to exploitation deep processing products of Chinese gall beihua, gallic acid was prepared with concentrated sulfuric acid catalytic hydrolysis of Chinese gall beihua in this paper. The influences of hydrolysis temperature, times and sulfuric acid dosage on the extraction ratio of gallic acid were studied by response surface methodology （RSM） based on single-factor experiment. The results showed that the extraction ratio of gallic acid from Chinese gall beihua is 36.01% with 50mL extraction solution under catalyzed with 2.47mL 98% concentrated sulfuric acid at 97℃ for 315min. Experiment results were well matched with the model predicted value. Employed with high performance liquid chromatography （HPLC） analysis, the results of the separation product are consistent in the standard substance. Theoretical basis was provided for the development and utilization of the Chinese gall beihua with the study.

关 键 词：倍花 没食子酸 响应面优化法 

分 类 号：TQ914.1[化学工程]