小瓜虫对草鱼肝脏和肾脏等组织器官线粒体的影响  

Effect of Ichthyophthirius multifiliis on Mitochondria of Liver and Kidney Tissues and Organs of Grass Carp

作　　者：陆英杰[1] 区炳庆[1] 许婉丽[1] 黄继兴 于辉[1] 

机构地区：[1]佛山科学技术学院,佛山528200

出　　处：《中国畜牧兽医》2015年第3期619-623,共5页China Animal Husbandry & Veterinary Medicine

基　　金：广东省科技攻关项目(2012A020602110)

摘　　要：本试验旨在了解小瓜虫(Ichthyophthirius multifiliis)感染草鱼后对草鱼肝脏、肾脏和肌肉等组织器官线粒体的影响。人工方法用小瓜虫感染健康草鱼,确定草鱼成功感染小瓜虫后,透射电镜观察肝脏、肾脏和肌肉组织中线粒体结构的变化,检测这些组织器官的过氧化物歧化酶(SOD)和丙二醛(MDA)含量。透射电镜观察结果显示,与正常组相比,感染组肾脏、肝脏和肌肉组织线粒体数量减少,线粒体肿胀、嵴断裂或消失。感染组肝脏、肾脏和肌肉组织中SOD含量平均值均高于正常组,其中肝脏、肾脏表现为差异显著(P<0.05),肌肉组织差异不显著(P>0.05);感染组肝脏、肾脏和肌肉组织中MDA平均含量均低于正常组,其中肝脏、肾脏表现为差异显著(P<0.05),肌肉组织差异不显著(P>0.05)。结果表明,小瓜虫感染草鱼后,对草鱼的肝脏、肾脏和肌肉组织的线粒体结构及SOD和MDA活性均有影响。The assay was aimed to understand the effect of Ichthyophthirius multifiliis on mitochondria of livers,kidneys and muscle tissues of grass carp.Health grass carps were infected by Ichthyophthirius multifiliis with artificial method,after successful infection,the mitochondria ultrastructure changes of livers,kidneys and muscle tissues of grass carp in the control and infection groups under the transmission electron microscope（TEM）were observed,contents of SOD and MDA were tested.The results showed that the quantities of the mitochondria decreased in the livers,kidneys and muscle tissues in infection group;The mitochondria of infection group swelled,ruptured or disappeared under the TEM compared with the control group;The contents of SOD in livers and kidneys in infection group were significantly higher than that of control group（P0.05）,the muscles＇ were not significantly different（P0.05）;Then the contents of MDA in livers and kidneys in infection group were significantly lower than that of control group（P0.05）,the muscles＇ were not significantly different（P0.05）.It could be seen that Ichthyophthirius multifiliis had effects on the mitochondria and contents of SOD and MDA in livers,kidneys and muscle tissues of grass carp after infection.

关 键 词：小瓜虫 草鱼 组织器官 线粒体 

分 类 号：S855.9[农业科学—临床兽医学]