Effects of Traditional Chinese Medicine on Numbers of Lymphocytes and Goblet Cells in Villus Epithelia of Layers under Heat Stress  

中药对热应激蛋鸡肠绒毛上皮内淋巴细胞和杯状细胞数量的影响（英文）

作　　者：史秋梅[1] 高桂生[1] 邢超[1] 刘欢[1] 沈萍[1] 潘方方[1] 高光平[1] 张艳英[1] 

机构地区：[1]河北科技师范学院,河北省预防兽医学重点实验室,河北秦皇岛066004

出　　处：《Agricultural Science & Technology》2015年第2期311-316,335,共7页农业科学与技术（英文版）

基　　金：Supported by National Natural Science Foundation of China(31472230);Hebei Provinceial Natrual Science Foundation of China(2014407068);Project of HebeiScience and Technology Department(14966610D,13826615D,12220408D)~~

摘　　要：[Objective] This study aimed to investigate the effects of traditional Chinese medicine additives on numbers of lymphocytes and goblet cells in villus epithelia of layers under heat stress.[Method] A total of 180 88-d-old healthy Esa Brown cocks were selected.They were randomly divided into 9 experimental groups：Room temperature control,High temperature control,VC addition group,High formula I,Moderate formula I,Low formula I,High formula II,Moderate formula II and Low formula II.The formula I and formula II were to add different herbal extracts to the diet of cocks with different doses.The cocks in the VC addition group were administered orally with same-concentration VC solution.After certain time,the cocks were slaughtered.Then the numbers of epithelial lymphocytes and goblet cells in various segments of small intestine were counted by using conventional histological section and HE staining.[Result] The numbers of lymphocytes and goblet cells in villus epithelia of layers under heat stress were decreased gradually with the proceeding of experiment.The herbal extracts of formula I and formula II all could promote the generation of lymphocytes and goblet cells.But the promoting effect of formula II was best.[Conclusion] The Chinese herbal medicine additives have a good relieving effect on heat stress in layers.[目的]旨在研究中草药添加剂对热应激蛋鸡小肠绒毛上皮内淋巴细胞和杯状细胞数量的影响。[方法]选择体质良好88日龄伊莎褐蛋公鸡180羽,随机分成9个试验组,分别为常温对照组、高温对照组、添加VC组、方一高、方一中、方一低、方二高、方二中、方二低剂量组。方一、方二分别灌服低、中、高三种剂量浓度的中草药提取物,添加VC组灌服同一浓度的VC水溶液。运用组织学常规切片技术和HE染色法,观察鸡小肠各肠段肠上皮内淋巴细胞和杯状细胞的数量。[结果]高温状态下鸡肠道黏膜上皮间淋巴细胞和杯状细胞的数量呈逐渐下降的趋势;方一、方二能促进淋巴细胞和杯状细胞生成,方二效果好于其他各组。[结论]添加中草药对于缓解蛋鸡的热应激有较好的效果。

关 键 词：Chinese herbal medicine Heat stress EPITHELIA LYMPHOCYTE Goblet cell 

分 类 号：S853.7[农业科学—临床兽医学]