应用454测序技术评价非酒精性脂肪性肝病患者肠道菌群结构差异  

Constitutional variation of gut microbiota detected by bar coded 454 pyrosequencing technology in patients with non-alcoholic fatty liver diseases

作　　者：沈峰[1] 陈建能 郑瑞丹 王晓颖[3] 潘勤[1] 陈光榆[4] 章瑞南[1] 徐雷鸣[1] 范建高[1] 

机构地区：[1]上海交通大学医学院附属新华医院消化内科,上海市200092 [2]漳州市正兴医院 [3]上海交通大学医学院附属新华医院病理科 [4]上海交通大学医学院临床流行病学研究中心

出　　处：《实用肝脏病杂志》2015年第2期145-149,共5页Journal of Practical Hepatology

基　　金：国家重点基础研究发展计划(973)项目(编号:2012CB517501);中国肝炎防治基金会"天晴肝病"研究基金(编号:20120027)

摘　　要：目的阐明非酒精性脂肪性肝病（NAFLD）患者肠道菌群结构特征。方法采用454焦磷酸测序技术,对47例经＂肝活检＂确诊的NAFLD患者和34例健康人新鲜粪便样品16S DNA V3-V5可变区进行测序,通过序列比较及操作分类单元（OTU）划分评价肠道菌群结构差异。结果 NAFLD患者OTUs为（88.32±28.27）,显著低于健康人[（109.65±30.65）,P〈0.01];在＂门＂水平,NAFLD患者厚壁菌门所占比例为（51.2±17.8）%,显著高于健康人的[（46.4±12.8）%,P=0.048],而拟杆菌门为（31.6±18.9）%,显著低于健康人的[（43.3±14.4）%,P〈0.001];在＂纲＂水平上,NAFLD组Erysipelotrichi纲占（3.2±5.1）%,显著高于健康人的[（1.0±1.2）%,P=0.009],而Bacteroidia纲占（31.0±18.8）%,显著低于对照组的[（42.3±14.0）%,P=0.004];在＂属＂水平上,NAFLD组乳球菌占（0.0038±0.0001）%,低于健康组的[（0.0145±0.001）%,P=0.003],普氏菌属也同样显著减少（P=0.022）,而链球菌（Streptococcus）在NAFLD占（1.50±0.03）%,显著高于对照组的[（0.21±0.24）%,P=0.004]。结论 NAFLD患者肠道菌群多样性减少,存在构成显著异常,是致病因素抑或其后果仍有待于进一步研究。Objective To elucidate the changes of gut microbiota projects in patients with non-alcoholic fat-ty liver diseases（NAFLD）. Methods Fresh faecal samples from 47 NAFLD patients underwent liver biopsies and 34 health individuals were examined using bar coded 454 pyrosequencing technology. 16S DNA V3-V5 variable regions were sequenced and the operational taxonomy unit （OUT） was analyzed to evaluate the differences of mi-crobiota projects. Results The OUT of patients with NAFLD were （88.32±28.27）,significantly lower than those in the controls [（109.65±30.65）,P〈0.01）];In Phylum level,the abundance of Firmicutes had significantly increased in NAFLD patients than in the controls [（51.2 ±17.8）% vs.（46.4 ±12.8）%,P=0.048],while the abundance of Bac-teroidetes had significantly reduced [（31.0 ±18.8）% vs.（43.3 ±14.4）%,P〈0.01];In Class level,the abundance of Erysipelotrichi had significantly increased in NAFLD patients than in the controls [（3.2±5.1）% vs. （1.0±1.2）%,P=0.009],while the abundance of Bacteroidia had significantly decreased [（31.0±18.8）% vs.（42.3±14.0）%,P=0.004];In Genus level,the Lactococcus [（0.0038±0.0001）%] had significantly reduced in NAFLD patients compared with controls [（0.0145±0.001）%,P=0.003];The same results were found in Prevotella Genus. However,Streptococcus had significantly increased in NAFLD patients [（1.50 ±0.03）%] than in controls （0.21 ±0.24）%,P=0.004]. Conclu-sions Patients with NAFLD had a lower diversity of gut microbiota projects.

关 键 词：非酒精性脂肪性肝病 肠道菌群 454焦磷酸测序 

分 类 号：R575[医药卫生—消化系统]