离子肼飞行时间质谱表征硫酸软骨素/硫酸角质素二糖  

Characterization of disaccharides from chondroitin / dermatan sulfate by ion trap time-of-flight hybrid mass spectrometry

作　　者：林江慧[1] 杜佳燕 付青[1] 魏峥[1] 

机构地区：[1]福州大学糖生物化学研究所,福州350002

出　　处：《分析试验室》2015年第3期270-274,共5页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金(21343013);国家基础科学人才培养基金项目(1103303)资助

摘　　要：硫酸软骨素(CS)和硫酸角质素(DS)结构决定它与蛋白的相互作用。因此对CS/DS结构的解析,能够进一步阐明结构与功能之间的关系。采用电喷雾离子阱多级质谱技术(ESI-MSn)在负离子模式(ESI-)下表征8种CS/DS二糖裂解规律。二糖的裂解发生在糖苷键和糖环位置,N-乙酰半乳糖胺上主要断裂方式为0,2X,而糖醛酸主要断裂方式为0,2Z。所含亚硫酸根越多越不稳定。亚硫酸根的位点决定了糖结构稳定性,含4位氧亚硫酸的二糖相对最不稳定。此外糖苷键的位置也决定了糖结构的稳定性。二级质谱表明同分异构体有其特定的裂解规律。该质谱裂解规律有助于CS/DS二糖同分异构体的鉴定。Chondroitin sulfate（ CS） / dermatan sulfate（ DS） structure determines its interaction with proteins,therefore the analysis of CS / DS can help further clarify structure and function relationships. Electrospray ionization-ion trap mass spectrometry（ ESI-MSn） were applied in negative mode. The characteristic（ ESI-MSn）fragmentation pattern of eight CS / DS disaccharides was discussed. The results show that the MS / MS fragment produces in glycosidic and cross-ring cleavage is observed. The0,2X fragment produces in N-acetylgalactosamine（ Gal NAc） while the0,2Z fragment yields in glucuronic acid. The position and quantity of sulfate groups in disaccharide determine its stability in MS,moreover,disaccharides containing C-4 sulfate are more unstable than others. In addition,the type of glycosidic has also effect on its stability. MS2 reveals that the disaccharide isomers have a characteristic fragment pattern. The results may contribute to identify the disaccharides isomer of CS / DS.

关 键 词：硫酸软骨素 硫酸角质素 二糖结构 离子阱时间飞行质谱 二级质谱 裂解规律 

分 类 号：O657.3[理学—分析化学]