HPLC法检测乳品中5-羟甲基糠醛和糠醛  

Detection of 5-Hydroxymethylfurfural and Furfural in Dairy Products by HPLC

作　　者：赵贞 李翠枝 岳虹 万鹏 吕海燕 邵建波 

机构地区：[1]内蒙古伊利实业集团股份有限公司,内蒙古呼和浩特010010

出　　处：《饮料工业》2015年第1期48-50,共3页Beverage Industry

基　　金："十二五"国家科技部支撑计划(2012BAK17B00)

摘　　要：建立了乳制品中5-羟甲基糠醛和糠醛的高效液相色谱分析方法。样品用沉淀剂沉淀过滤后,以水-甲醇溶液为流动相,C18反相色谱柱分离,紫外检测器280nm检测。结果表明,5-羟甲基糠醛和糠醛可以完全分离。在0.03~100.00mg/kg范围内,该方法呈良好的线性关系,回收率为83.3%~113.3%。本方法用于乳制品中5-羟甲基糠醛和糠醛的测定,操作简单、定量准确、重复性好。A method for detecting of 5-Hydroxymethylfurfural（5-HMF） and furfural in dairy products by HPLC is described. The samples were precipitated and filtered at first, then separated on a C18 column with the mobile phase（water-methanol solution）,and detected with UV detector at 280 nm. The result showed that 5-HMF and furfural were completely separated. The method has good linearity relationship in the range of（0.03~100）mg/kg,and high recovery rate at（83.3~113.3）%. This method is simple, accurate and good reproducibility for detecting of 5-HMF and furfural in dairy products.

关 键 词：液相色谱法 5-羟甲基糠醛 糠醛 乳制品 

分 类 号：TS252.7[轻工技术与工程—农产品加工及贮藏工程] O657.72[轻工技术与工程—食品科学与工程]