隐丹参酮对人胃癌SGC-7901细胞增殖及血管内皮生长因子mRNA表达的影响  

Effect of cryptotanshinone on the proliferation and vascular endothelial growth factor mRNA expression in human gastric cancer cell line SGC-7901

作　　者：邓凤春[1] 于占江[2] 杨钰[2] 赵红晔[3] 王滨[3] 周丽[3] 

机构地区：[1]齐齐哈尔医学院解剖教研室,黑龙江齐齐哈尔161006 [2]齐齐哈尔医学院第三附属医院普外科,黑龙江齐齐哈尔161001 [3]齐齐哈尔医学院生理教研室,黑龙江齐齐哈尔161006

出　　处：《中国医药导报》2015年第6期7-10,共4页China Medical Herald

基　　金：黑龙江省教育厅科学技术研究项目(编号12531830)

摘　　要：目的通过测定隐丹参酮对人胃癌SGC-7901细胞增殖及血管内皮生长因子（VEGF）mRNA的影响,探讨其抗肿瘤的作用机制。方法采用四甲基偶氮唑蓝（MTT）比色法检测隐丹参酮对人胃癌SGC-7901细胞增殖的影响,采用Real Time RT-PCR检测隐丹参酮对VEGF m RNA表达的影响。结果 20、40、60、80、100μmol/L的隐丹参酮作用于人胃癌SGC-7901细胞6-48 h,其生长和增殖均受到一定程度的抑制;24 h为隐丹参酮对SGC-7901细胞抑制作用的最佳时间,IC50为59.11μmol/L;选取40、60和80μmol/L 3个剂量作用于人胃癌细胞SGC7901 24 h后,60和80μmol/L的隐丹参酮均可下调VEGF m RNA的表达（均P〈0.01）。结论隐丹参酮可抑制人胃癌SGC-7901细胞增殖,并能抑制VEGF mRNA的表达,提示这可能是其抗肿瘤的作用机制之一。Objective To observe the effect of cryptotanshinone on cell proliferation and vascular endothelial growth factor（VEGF） m RNA expression in human gastric cancer cell line SGC-7901, and to explore its possible anti-tumor mechanism. Methods Human gastric cancer SGC-7901 cells were cultured in vitro and treated with cryptotanshinone.The cell proliferation was examined by the method of MTT. The transcriptions of VEGF m RNA were demonstrated by real-time RT-PCR. Results The cryptotanshinone groups with 20, 40, 60, 80, 100 μmol/L five different concentrations had significant inhibitory effects on the proliferation of human gastric cancer SGC-7901 cells in 6, 12, 24 h and 48 h after drug action. This effect increased with increasing drug concentration and action time. 24 h was the best time of cryptotanshinone action, and IC50 of 24 h was 59.11 μmol/L. In the following study, the effects of cryptotanshinon groups with 40, 60, 80 μmol/L three different concentrations on human gastric cancer SGC-7901 cells were observed,the results indicated that 60 and 80 μmol/L cryptotanshinon down-regulated the expression of VEGF m RNA（both P 〈0.01）. Conclusion Within a certain drug concentration, cryptotanshinone can inhibit the proliferation of SGC-7901 cells and plays a potential role in inhibiting angiogenesis by down-regulating the expression of VEGF m RNA.

关 键 词：隐丹参酮 人胃癌SGC-7901细胞 四甲基偶氮唑蓝 血管内皮生长因子 实时荧光定量PCR 

分 类 号：R735.2[医药卫生—肿瘤] R285.5[医药卫生—临床医学]