氧化应激在姜黄素诱导肺癌细胞凋亡中的作用  

Role of Oxidative Stress on Curcumin Induced Lung Cancer Cell Apoptosis

作　　者：林妙[1] 姚庆华[2] 汪玉琪[2] 陈良良[2] 

机构地区：[1]浙江中医药大学第一临床医学院,浙江杭州310053 [2]浙江省肿瘤医院,浙江杭州310022

出　　处：《中国肿瘤》2015年第3期229-233,共5页China Cancer

摘　　要：[目的]探讨姜黄素诱导肺癌A549细胞凋亡中氧化应激的作用。[方法]将不同浓度（5~40μM）的姜黄素作用于肺癌细胞株A549细胞，以MTT法检测姜黄素不同浓度（5~40μM）在不同时间点（3、6、12、24、48h）对A549细胞的抑制增殖率，流式细胞术检测细胞凋亡率：DCFH—DA探针染色流式检测细胞内活性氧（reactive oxygen species，ROS）的变化，硫代巴比妥酸法检测丙二醛（malondialdehvde，MDA），Westernblot检测Bcl-2、Bax和HSP70的表达。[结果]20μM姜黄素作用细胞12h和24h时，细胞增殖抑制率分别为32．17％和52．16％。姜黄素对A549细胞的生长抑制作用呈剂量和时间依赖性。A549细胞凋亡率和坏死率随着姜黄素的浓度增高而增加。经姜黄素处理后，细胞内活性氧显著性降低，丙二醛活性下降，HSP70活性下降，Bax／Bel-2比值增高。[结论]姜黄素可调节肺癌细胞内氧化还原状态，从而促进肿瘤细胞凋亡。[Purpose] To investigate the effect of curcumin on the proliferation and apoptosis in A549 lung cancer cells. [Methods] Different concentrations （5-40μM） of curcumin treated with A549 cells in different times （3,6,12,24,48h）. The cell viability was determined by MTF assay. The apoptosis was detected by flow cytometry. The level of reactive oxygen species （ROS） was de- termined with DCFH-DA by flow eytometry, and malondialdehyde（MDA） was detected by malondi- aldehyde barbiturie acid method. Bcl-2,Bax,HSPT0 activity was assayed by Western blot. [Re- sults] 20μM curcumin treated with cell for 12h and 24h,the cell proliferation inhibition rate was 32.17% and 52.16% ,respectively. The inhibitory effect of cureumin on A549 cells was in a dose- and time-dependent manner. Apoptosis and necrosis rate of A549 cells increased with concentra- tion of curcumin. After curcumin treated,intracellular ROS significantly reduced,MDA activity declined,then HSPTO activity declined,Bax/Bcl-2 ratio increased. [Conclusion] Cureumin could regulate intraeellular REDOX state and promote the tumor cell aoootosis in lung cancer.

关 键 词：姜黄素 肺癌 凋亡 氧化应激 热休克蛋白70 

分 类 号：R734.2[医药卫生—肿瘤]