慢病毒介导Stathmin-1基因沉默对宫颈Hela细胞p53蛋白的影响  

Lentivirus-mediated Stathmin-1 Silencing on p53 Protein in Cervical Hela Cells

作　　者：孔守芳[1] 戴淑真[2] 吕腾[2] 袁汇[1] 孙选[1] 钟金妍[1] 

机构地区：[1]青岛大学医学院附属青岛市海慈医疗集团,山东青岛266033 [2]青岛大学附属医院黄岛院区,山东青岛266000

出　　处：《中国肿瘤》2015年第3期246-249,共4页China Cancer

基　　金：青岛市科技发展计划(13-1-3-118-jcl)

摘　　要：[目的]研究宫颈癌Hela细胞中Stathmin-1表达对p53蛋白的影响及对细胞增殖的作用。[方法]构建Stathmin-1 siRNA慢病毒载体,包装重组慢病毒,感染宫颈癌Hela细胞株,沉默Stathmin-1表达为干扰组,采用导入阴性片段的慢病毒感染Hela细胞作为对照组。MTT法检测细胞增殖,蛋白印迹法(Western-blot)检测细胞p53蛋白的表达。[结果 ]感染重组慢病毒及阴性病毒后,Stathmin-1基因干扰组Hela细胞增殖24h后较对照组抑制明显(P<0.05)。与对照组相比,干扰组中p53蛋白表达明显减少(P<0.05)。[结论]宫颈癌Hela细胞中Stathmin-1影响p53蛋白表达,沉默Stathmin-1可抑制细胞增殖。[Purpose] To study the influences Stathmin-1 on p53 protein expression in Hela cells and the role on cells proliferation. [Methods] Short hairpin RNA（shRNA） targeting Stathmin-1 was designed and lentivirus carried the shRNA infected Hela cells as interference group. Control group cells were infected with lentivirus carried negative fragment. After recombinant ]entivirus infected Hela cells, MTT assay was used to detect the status of proliferation. Western blot was used to ana- lyze p53 protein expression. [Resuhs] With STMN-1 silencing,MTT assay results suggested Hela cells proliferation was suppressed obviously in interference group compared with control group （P〈 0.05）.The p53 protein expression in interference group was decreased （P〈0.05）. [Conclusion] Stathmin-1 expression can influence p53 protein expression in cervical Hela cells. Silencing Stath- min-1 can restrain cervical cancer cells proliferation.

关 键 词：Stathmin-1 P53蛋白 HELA细胞 慢病毒 细胞增殖 

分 类 号：R737.33[医药卫生—肿瘤]