肿瘤干细胞标志物在鼻咽癌细胞株中的表达  

The expression of cancer stem cell markers in human nasopharyngeal carcinoma cell lines

作　　者：刘观成[1] 何晓松[2] 宣广旭[2] 王文华[2] 陈文文[1] 

机构地区：[1]桂林医学院,广西桂林541004 [2]桂林医学院附属医院耳鼻喉头颈外科,广西桂林541001

出　　处：《现代医药卫生》2015年第6期801-803,共3页Journal of Modern Medicine & Health

摘　　要：目的探索肿瘤干细胞(CSC)标志物ABCG2、CD44、CD34在鼻咽癌细胞株CNE2、5-8F和6-10B中的表达情况,为选择CSC标志物进行鼻咽癌CSC样研究奠定基础。方法常规培养CNE2、5-8F和6-10B这3种鼻咽癌细胞株,用流式细胞仪检测3种鼻咽癌细胞株中ABCG2、CD44、CD34细胞比例及其交互情况。结果 ABCG2在CNE2、5-8F和6-10B这3种鼻咽癌细胞株中表达率分别为0.1%、18.6%、19.9%;CD44在CNE2、5-8F和6-10B中表达率分别为99.5%、93.2%、99.1%;CD34在CNE2、5-8F和6-10B中表达率分别为0、3.0%、0.1%。在5-8F中,ABCG2+CD44+细胞交互比例为11.6%,ABCG2+CD34+细胞交互比例为0。结论 5-8F和6-10B中CD34+细胞比例与CSC理论中CSC的比例相符。CD34可以作为鼻咽癌CSC的候选标志物。Objective To explore the expression of cancer stem cells（CSC） markers named ABCG2,CD44,CD34 in hu man nasopharyngeal carcinoma（NPC） CNE-2,5-8F and 6-10B cell lines to provide the basis of CSC markers research of NPC. Methods The NPC cell lines of CNE2,5-8F and 6-10B were cultivated regularly,of which,ABCG2,CD44,CD34 cell ratio was detected with flow cytometry. Results ABCG2 positive cells accounted about 0.1%,18.6%and 19.9%in the CNE-2,5-8F,6-10B cell lines. CD44 positive cells accounted 99.5%,93.2%and 99.1%in the CNE-2,5-8F and 6-10B cell lines. CD34 positive cells accounted 0,3.0%and 0.1%in the CNE2,5-8F,and 6-10B cell lines. ABCG2^＋CD44^＋cell interation accounted about 11.6%and ABCG2^＋CD44^＋cell interation accounted about 0 in 5-8F cell lines. Conclusion The expression proportion of CD34^＋in the 5-8F and 6-10B cell lines confirms to CSC markers of the CSC theory. CD34 may be deemed as a candidate marker of nasopharyn-geal neoplas.

关 键 词：肿瘤干细胞 生物学标记 鼻咽肿瘤/病理学 细胞系 表达 交互作用 

分 类 号：R739.63[医药卫生—肿瘤]