博落回内生放线菌的分离及活性菌株的鉴定  

Isolation and Identification of an Antimicrobial Endophytic Actinomycete from Macleaya cordata

作　　者：汪学军[1,2] 闵长莉[1,2] 殷智超[1] 杨艳[1] 

机构地区：[1]皖西学院生物与制药工程学院,安徽六安237012 [2]皖西学院大别山植物内生真菌资源研究中心,安徽六安237012

出　　处：《中药材》2014年第11期1947-1950,共4页Journal of Chinese Medicinal Materials

基　　金：国家自然科学基金(31100019);安徽省高等学校省级自然科学研究重点项目(KJ2013A266)

摘　　要：目的:从博落回Macleaya cordata(Willd.)R.Br根部筛选具有抑菌活性的放线菌,并对其进行种属鉴定。方法:采用平板涂布法分离皖西大别山野生植物博落回根部的内生放线菌;采用琼脂块法初步研究博落回内生放线菌的抑菌活性;通过形态特征、生理生化特性及分子生物手段鉴定菌种。结果:从博落回根部共分离筛选得到内生放线菌15株,有6株放线菌对供试菌株表现出抑菌活性,占总分离菌株的40%,其中菌株BL7对金黄色葡萄球菌与枯草芽孢杆菌的拮抗作用明显,其抑菌圈直径分别为29、20 mm。根据菌株BL7的形态学特征、生理生化特征并结合16S r DNA系统发育分析将其初步鉴定为茂原链霉菌Streptomyces mobaraensis。结论:链霉菌属放线菌在医药上具有广阔的应用前景。而茂原链霉菌Streptomyces mobaraensis首次从博落回植株中筛选得到,具有进一步研究开发的价值。Objective： An antimicrobial endophytic actinomycete strain was isolated from the root of Macleaya cordata,and its phylogenetic position was also investigated. Methods： Endophytic actinomycetes were isolated from the root of Macleaya cordata growing in Dabie Mountain by tablet coating method. Antimicrobial activities of endophytic actinomycetes were determined by the agar block method,and then a potential strain was identified by morphology,biochemical characterization and molecular methods. Results： A total of 15 strains of endophytic actinomycetes were isolated from the samples,six of them had antimicrobial activity to the test strains which accounts for 40% of all strains. Among the six strains,the strain BL7 was found to produce antibiotic substances which showed significant inhibitory effects on Staphylococcus aureus and Bacillus subtilis,with a formed inhibition zone of 29 mm and 20 mm respectively. Based on the morphology,biochemical characterization and 16 SrDNA sequence analysis,the strain BL7 was preliminary identified as Streptomyces mobaraensis. Conclusion： Some members of Streptomyces genus have a very wide application prospect in medicine. This is the first report that Streptomyces mobaraensis strain was isolated from Macleaya cordata,and this study provides basis for the further exploration of the strain.

关 键 词：博落回 内生放线菌 抑菌活性 分离 茂原链霉菌 

分 类 号：R282.2[医药卫生—中药学]