甲啶铂在玻碳电极上的电化学行为及其与DNA相互作用研究  

Study on the Electrochemical Behavior of Picoplatin and Interaction of Picoplatin with DNA at Glassy Carbon Electrode

作　　者：叶丽清[1] 闫莉莉[1] 向明武 高云涛[1] 贝玉祥 

机构地区：[1]云南民族大学化学与生物技术学院,民族药资源化学国家民委-教育部重点实验室,昆明650500 [2]昆明贵研药业有限公司,昆明650106

出　　处：《贵金属》2014年第1期63-69,共7页Precious Metals

基　　金：云南省创新团队计划(2011HC008);云南省高校科技创新团队计划项目(2011UY09)

摘　　要：采用循环伏安法（CV）和差分脉冲伏安法（DPV）,研究了甲啶铂在多壁碳纳米管-离子液体修饰玻碳电极（MWNTs-[ODMIM]PF6/GCE）上的电化学行为,建立了甲啶铂的测定方法;以紫外-可见光谱法和电化学方法相结合,研究了甲啶铂与DNA的嵌插结合作用。结果表明：甲啶铂在MWNTs-[ODMIM]PF6/GCE上有一对氧化还原峰,氧化峰电位Epa与还原峰电位Epc分别为-0.07 V、-0.36 V,峰电位之差为ΔE=0.29 V,Ipa/Ipc=1.11。在优化条件下,甲啶铂的氧化峰电流与其浓度在2.66-532μmol/L范围内成良好的线性关系（r=0.9994）,检出限为1.33μmol/L。方法操作简便,准确可靠、灵敏度高,可用于甲啶铂含量的直接测定。The electrochemical behavior of picoplatin at multi-walled carbon nanotubes ionic liquids modified glassy carbon electrode（MWNTs-[ODMIM]PF6/GCE） was investigated by cyclic voltammetry and differential pulse voltammetry, and the method of picoplatin determination was established. The mode of intercalation between picoplatin with DNA was studied by electrochemical methods combined with UV-visible spectroscopy. The results show that one pair of redox peaks of picoplatin was obtained at MWNTs-[ODMIM]PF6/GCE by cyclic voltammetry with-0.07 V of Epa and-0.36 V of Epc, ΔE=0.29 V, and Ipa/Ipc=1.11. Under optimal conditions, the oxidization peak current was linearly related to the concentration of picoplatin in the range of 2.66~532 μmol/L with the detection limit of 1.33 μmol/L. The method was convenient, accurate, credible and sensitive, it can be used in the direct determination of picoplatin.

关 键 词：分析化学 甲啶铂 多壁碳纳米管 离子液体 玻碳电极 电化学行为 伏安法 

分 类 号：O657.1[理学—分析化学]