棘孢曲霉利用蚕沙发酵产果胶酶的条件优化研究  

Study on the optimum condition of pectinase production by Aspergillus aculeatus with silkworm faeces fermentation

作　　者：何海燕[1] 黎凤华[1] 涂新凤 覃拥灵[1] 

机构地区：[1]河池学院化学与生物工程学院,广西高校微生物及植物资源开发利用重点实验室,宜州546300

出　　处：《中国食品添加剂》2015年第3期92-96,共5页China Food Additives

基　　金：生物化工广西重点学科(河池学院)建设基金(201308);河池学院重点科研课题(2013ZA-N002)

摘　　要：果胶酶是能够分解果胶物质的多种酶类的统称。本研究以前期选育所得高产果胶酶菌株棘孢曲霉(Aspergillus aculeatus)DW75作为实验菌株,通过蚕沙固体发酵,对产果胶酶的发酵条件进行了优化。通过单因素试验和正交试验得出了该菌株的最佳产酶条件为:发酵培养基初始p H值5.0,最佳氮源硫酸铵添加量0.1%,培养基料水比(W/V)1.8∶2,发酵温度25℃,发酵时间144h。在此优化发酵条件下菌株的产酶能力可达到19.146U/m L,是优化前酶活10.166U/m L的1.88倍。Pectinases are group enzymes which can breakdown pectin. In this experiment,silkworm faeces was used as fermentation material for pectinase production with Aspergillus aculeatus DW75. After the single factor and orthogonal design experiments,the optimal combinations were confirmed as follow ：Initial p H of medium was 5.0,the Ammonium sulphate content was 0.1%,ratio of medium material and water was 1.8 ∶ 2（W/V）,and 25 ℃ cultured for about 144 h. Under the optimized conditions,the Enzyme activity was 19.146U/m L,88% higher than the initial fermentation conditions.

关 键 词：棘孢曲霉 蚕沙 果胶酶 正交设计 

分 类 号：Q939.97[生物学—微生物学]