冬凌草甲素和survivin反义核苷酸对前列腺癌细胞作用的研究  

Effects of survivin antisense oligodeoxynecleotides and Oridonin on PC-3 cells

作　　者：李进 杨罗艳[2] 吴洪涛[2] 

机构地区：[1]湘潭市中心医院泌尿外科,湖南湘潭411100 [2]中南大学湘雅二医院泌尿外科

出　　处：《中国男科学杂志》2014年第12期11-15,共5页Chinese Journal of Andrology

摘　　要：目的：探讨冬凌草甲素联合survivin反义核苷酸（反义链）对前列腺癌PC-3细胞株增殖和凋亡以及survivin mRNA和蛋白的影响。方法常规培养PC-3细胞，用四甲基偶氮唑盐法（MTT法）检测survivin反义链联合冬凌草甲素对PC-3细胞增殖的影响；流式细胞仪（FCM）检测PC-3细胞凋亡率；以CalcuSyn药效学软件计算联合指数（CI）评价survivin反义链联合凌草甲素对PC-3细胞的联合效应，并通过荧光定量PCR和Western blot方法检测PC-3细胞survivin基因和蛋白表达变化。结果 survivin反义链转染PC-3细胞后，可以显著抑制PC-3细胞增殖，且能诱导PC-3细胞凋亡；冬凌草甲素联合survivin反义链对PC-3细胞增殖抑制率较两者单用组明显增强，显示出协同效应（Fa＜0.8）；冬凌草甲素和survivin反义链联用对PC-3细胞凋亡诱导作用更为显著（P＜0.01）；荧光定量PCR及Western blot显示反义链和冬凌草甲素均使survivin mRNA和蛋白表达下降，两者联合使survivin mRNA和蛋白表达下降更明显（P＜0.01）。结论 survivin基因反义链和冬凌草甲素均能明显抑制PC-3增殖、诱导细胞凋亡和下调survivin基因和蛋白表达；两者联合作用有协同效应。Objective To explore the synergistic effects of survivin antisense oligonucleotides combined with Oridonin on growth, apoptosis, and the expression of survivin of PC-3 cells. Methods Human prostate carcinoma cells PC-3 on logarithmic growth phase were used in this study. The cell vitality was determined by MTT assay. The combination index （CI） was calculated using Pharmaconamics CalcuSynsoftware. The apoptotic rate was examined by flow cytometer （FCM）. The expression of survivin was detected by Western Blot and Real-time Fluorescent Quantitation-PCR. Results After transfection with antisense Survivin RNAi, the proliferation of PC-3 cells was inhibited markedly. An obvious apoptosis was found in the transfected PC-3 cells. The inhibitory effect of combined administration of survivin antisense and Oridonin on cell proliferation was much stronger than that of the single way （P〈0.01）. It showed that there was a synergistic effect （Fa〈0.80）. Western Blot and RT-PCR assays demonstrated that survivin antisense and Oridonin all inhibited the expression of survivin（P 〈0.01）. Conclusion Combined survivin antisense and Oridonin significantly inhibits cell proliferation, induces cell apoptosis and down-regulates survivin expression in PC-3 cells, indicating that survivin antisense and Oridonin have a synergistic effect on PC-3 cells.

关 键 词：冬凌草素 前列腺肿瘤 细胞系 肿瘤 细胞增殖 细胞凋亡 

分 类 号：R737.25[医药卫生—肿瘤]