川芎嗪纳米制剂对大鼠腹膜间皮细胞保护作用的实验研究  

Experimental study on protection of tetramethylpyrazine nanoparticles on rat peritoneal mesothelium cells

作　　者：朱燕[1] 曾莉[2] 李文林[2] 毛春芹[3] 王晓雯[3] 

机构地区：[1]南京中医药大学第一临床医学院,江苏南京210023 [2]南京中医药大学图书馆,江苏南京210023 [3]南京中医药大学药学院,江苏南京210023

出　　处：《中国现代医学杂志》2015年第7期24-27,共4页China Journal of Modern Medicine

基　　金：江苏省中医药局科技项目(No:LZ13006);南京中医药大学青年自然科学基金(No:13XZR09)

摘　　要：目的研究川芎嗪纳米制剂对大鼠腹膜间皮细胞的保护作用。方法胰酶消化法体外培养出大鼠腹膜间皮细胞(RPMCs),免疫组化进行鉴定;MTT法测定川芎嗪纳米喷雾剂对经TNF-α诱导大鼠腹膜间皮细胞的活力的影响。结果培养出的细胞经鉴定为RPMCs;经TNF-α诱导的RPMCs活力显著下降,而川芎嗪纳米制剂能够抑制经TNF-α诱导的RPMCs活力下降,对RPMCs有一定的保护作用。结论川芎嗪纳米制剂通过保护RPMCs而发挥其抗粘连作用。【Objective】To study protective effect of tetramethylpyrazine nanoparticles on rat peritoneal mesothelial cells.【Methods】After enzyme digestion in vitro cultured rat peritoneal mesothelial cells（RPMCs） were identified by immunohistochemistry as RPMCs; MTT method was used to detect the effect of tetramethylpyrazine nanoparticles on the availability of TNF-α-induced rat peritoneal mesothelial cells.【Results】The cultured cells were identified as RPMCs; TNF-α-induced RPMCs activity significantly decreased, while tetramethylpyrazine could inhibit TNF-α-induced RPMCs activity decrease and had protective effect on RPMCs. 【Conclusion】Tetramethylpyrazine nanoparticles exert its anti-adhesion effect via protecting RPMCs.

关 键 词：川芎嗪 纳米制剂 腹膜间皮细胞 原代细胞 腹腔粘连 

分 类 号：R-332[医药卫生]