中药铁皮石槲对D-半乳糖加亚硝酸钠致老年痴呆症小鼠模型的影响  

Effect of Chinese Iron Ore Plant on Alzheimer＇ s Disease Mice Model Induced by D- galactose and Sodium Nitrite

作　　者：何胜[1] 邓晓珍[1] 潘琳[1] 林美情 宁梅[1] 钟涓 韦健全[1] 张树球[1] 

机构地区：[1]右江民族医学院重金属与氟砷毒物研究实验室,中国百色533000

出　　处：《国际老年医学杂志》2015年第2期49-52,85,共5页International Journal of Geriatrics

基　　金：广西壮族自治区教育厅科研基金（2013YB188）

摘　　要：目的：探讨铁皮石槲对D-半乳糖加亚硝酸钠致老年痴呆症（AD）小鼠模型的影响。方法：用D-半乳糖加亚硝酸钠给小鼠作腹腔注射60d，建立拟老年痴呆症模型，将造模小鼠分成四组：实验1组、实验2组、实验3组、模型组，另设一个不造模的正常组为正常对照。造模到25d后，实验组开始灌药治疗，实验1组用中药铁皮石槲、实验2组用首乌复方、实验3组用金银花复方水提取液灌胃，模型组和正常组用等体积蒸馏水代替，至实验结束前1周停药。实验过程前、中、后，用水迷宫试验测定小鼠游水时间，判断记忆力变化。实验结束，取小鼠血清和大脑，测定血清和脑匀浆的生化指标。结果：正常组、实验1组、实验2组、实验3组、模型组，脑匀浆乙酰胆碱酯酶（AchE）活力依次为1．50±0．07、1．88±0．19、1．70±0．21、1．72±0．23、1．76±0．20（U／mgprot），正常组明显低于实验1组和模型组，P〈0．05，P〈0．01；脑匀浆谷胱甘肽过氧化物酶（GSH—PX）活性依次为14．68±1．08、14．87±1．99、14．53±2．41、13．35±2．24、9．82±3．16活力单位（U／mgprot），模型组明显低于其他各组，P〈0．01，差异有统计学意义；脑匀浆丙二醛（MDA）含量依次为9．78±0．89、10．88±1．36、9．29±0．94、11．17±1．07、11．36±0．64（nmol／mgprot），正常组、实验2组明显低于其他三组（P〈0．05）；血清尿素氮，模型组明显高于其他各组，P〈0．05或P〈0．01，差异有统计学意义；水迷宫测定结果，水迷宫时间，造模组造模后水迷宫游水时间明显延长了，但实验组治疗后水迷宫游水时间缩短，错误率也下降，而正常组和模型组前后变化不明显。结论：中药铁皮石槲与对照药首乌复方，金银花复方水提取液对D-半乳糖加亚硝酸钠致老年痴呆症（AD）小鼠模型均有-定的抗衰老作用。OBJECTIVE ： To investigate the effect of Chinese iron ore plant on Alzheimer＇ s disease mice model induced by D - galactose and sodium nitrite. METHODS ： D - galactose sodium nitrite was used in the mice by intraperitoneal injection for 60 days. The model mice were divided into 4 groups ： experimental group 1, experimental group 2, experimental group 3 and the model group. The normal group was established and it is not a model group. Molding to 25 days, the experimental group was treated by Chinese iron ore plant, experimental group 2 used Radix Polygoni Multiflori compound, experimental group 3 received honeysuckle compound water extract gastric perfusion, model group and normal group accepted equal volume of distilled water. The drugs used in all of the groups were withdrawn at the end of the experiment. During the experimental process swimming time of mice was tested by water maze test and the memory changes were judged. At the end of the experiment, serum and brain of mice were taken and biochemical index of blood and brain homogenate of the qing were tested. RESULTS ： In the normal group, experimental group 1, experimental group 2, experimental group 3 and the model group, brain acetylcholinesterase （AchE） activity was 1. 50 ± 0. 07, 1.88 ± 0. 19, 1.70 ± 0. 21, 1.72 ±0. 23, 1.76 ±0. 20 （U/mgprot） respectively. The indexes aboved in normal group were obviously lower than those in experiment 1 group and model group （P 〈 0. 05, P 〈 0. 01 ）. Brain homogenate glutathione peroxidase （GSH-PX） activity was 14. 68 ± 1.08, 14. 87± 1.99, 14. 53 ± 2. 41, 13.35 ± 2. 24, 9. 82 ± 3.16 unit of activity （U/mgprot） respectively in these groups. The index in the model group was significantly lower than that in other groups （ P 〈 0. 01 ）. There was statistically significant difference. Brain homogenate C two aldehyde （MDA） content was 9. 78 ±0.89, 10.88±1.36, 9.29±0.94, 11.17 ±1.07, 11.36±0.64 （nmol/mgprot） respectively in these groups. The index in normal group and

关 键 词：铁皮石槲 老年痴呆症 抗衰老作用 

分 类 号：R749.16[医药卫生—神经病学与精神病学]