雷帕霉素对膀胱癌5637细胞增殖及细胞周期的影响  

Effect of Rapamycin on Proliferation and Cell Cycle of Human Bladder Carcinoma Cell Line 5637

作　　者：单广夷[1] 付成[1] 陈昂[1] 胡滨[1] 毕缓[1] 

机构地区：[1]辽宁省肿瘤医院泌尿外科,沈阳110024

出　　处：《中国医科大学学报》2015年第3期230-233,共4页Journal of China Medical University

基　　金：辽宁省自然科学基金(20092028)

摘　　要：目的探讨雷帕霉素对膀胱癌5637细胞增殖能力和细胞周期的影响。方法 5637细胞贴壁培养于含10%胎牛血清、100 U/m L青霉素、100μg/m L链霉素的RPMI 1640培养液中,37℃、5%CO2饱和湿度培养箱中培养。处理组加入50、100、200和400 nmol/L的雷帕霉素,对照组不加药物,每天更换RPMI 1640培养液。MTT法检测处理组的生长抑制率,流式细胞术检测不同浓度的雷帕霉素对5637细胞增殖周期的影响。结果与对照组相比,雷帕霉素对5637细胞有明显抑制作用,呈浓度依赖性和时间依赖性。雷帕霉素可以将5637细胞周期阻滞在G0/G1期,从而抑制细胞的增殖,且未见明显的凋亡情况。结论雷帕霉素使5637细胞阻滞在G0/G1期,抑制了膀胱癌5637细胞的增殖,说明雷帕霉素的作用靶点参与了5637的增殖过程,其有望成为膀胱癌治疗的新靶点。Objective To evaluate the effect of rapamycin on proliferation and cell cycle of human bladder cancer cell line 5637. Methods Total？ly 5637 cells were maintained in RPMI 1640 containing 10%fetal bovine serum,100 U/mL penicillin and 100μg/mL streptomycin for adherent cul？ture. Cells were grown at 37℃in a 5%CO2 incubator. The 5637 cells were treated with various concentrations of rapamycin solution（50 nmol/L, 100 nmol/L,200 nmol/L,and 400 nmol/L）. The control group was daily treated with single RPMI 1640 without medication. The growth repression rate for 5637 cells was evaluated by MTT. Flow cytometry was used to investigate the effect of rapamycin of different concentrations on cell cycle of 5637 cells. Results Compared to the control group,rapamycin can inhibit the proliferation of 5637 cells in a concentration and time dependent manner. Rapamycin inhibited 5637 cells at G0/G1 thus inhibiting cell proliferation but not inducing apoptosis. Conclusion Rapamycin inhibited growth of 5637 bladder carcinoma cells and arrested cell cycle at G0/G1,indicating that mammalian target of rapamycin may play an important role in proliferation of 5637 cells and act as a new tumor therapeutic target in patients with bladder cancer.

关 键 词：雷帕霉素 5637细胞 增殖 细胞周期 

分 类 号：R737.14[医药卫生—肿瘤]