加巴喷丁对大鼠坐骨神经慢性压迫损伤DRG中TRPV1的影响  

The Effect of Gabapentin on TRPV1 in Rat DRG after CCI

作　　者：阎妮[1] 栾永[1] 李润玖[2] 

机构地区：[1]大连医科大学附属第一医院麻醉科,大连116011 [2]大连医科大学附属第一医院急诊科,大连116011

出　　处：《中国医科大学学报》2015年第3期234-237,共4页Journal of China Medical University

基　　金：辽宁省教育厅科学研究一般项目(L2013351)

摘　　要：目的研究加巴喷丁对坐骨神经慢性压迫损伤(CCI)大鼠DRG中TRPV1的影响。方法使用SD雄性大鼠建立CCI模型,随机分为3组:对照组,CCI组,CCI+GBP组。使用行为学检测机械性痛和热痛。术后第8天,搜集DRG,用RT-PCR和Western blot检测DRG中TRVP1的表达情况。结果 CCI手术后DRG中TRPV1基因的m RNA表达显著增加,GBP能够减少CCI后DRG的TRPV1基因的m RNA表达。结论在CCI手术后,DRG中TRPV1基因的表达升高,使用GBP后,其表达降低。Objective To study the effect of Gabapentin（GBP）on the transient receptor potential vanilloid l（TRPV1）in dorsal root ganglion （DRG）of rats with chronic constriction injury（CCI）. Methods SD male rats were used to establish CCI model and randomly divided into 3 groups,the control group,the CCI group,and the CCI＋GBP group. Mechanical allodynia and thermal hyperalgesia were measured to evaluate the es-tablishment of CCI model and the analgesic effect of GBP. At the postoperative 8 days,all rats were sacrificed and dorsal root ganglions were collect-ed for further RT-PCR and Western-blotting analysis to detect expression of TRVP1 in DRG. Results The mRNA and protein expression of TRPV1 were both significantly increased in DRG after CCI operation and GBP could decrease mRNA expression of TRPV1 gene in DRG after CCI. Conclusion The expression of TRPV1 was increased in DRG after CCI operation which was decreased after administration of GBP.

关 键 词：神经痛 加巴喷丁 坐骨神经慢性压迫损伤 大鼠 TRPV1 

分 类 号：R745.4[医药卫生—神经病学与精神病学]