微小RNA-200a在肝癌患者血清及癌组织中的表达及其与预后的关系  

The expressions of microRNA-200a in serum and carcinoma tissues and its relationship with prognosis in patients with hepatocellular carcinoma

作　　者：龚哲[1] 尚闯[2] 单国用[1] 刘兴安[1] 

机构地区：[1]郑州人民医院放疗科,450000 [2]郑州大学附属肿瘤医院普外科

出　　处：《中华实验外科杂志》2015年第3期608-610,共3页Chinese Journal of Experimental Surgery

摘　　要：目的 探讨肝癌患者血清及癌组织中微小RNA（miRNA,miR）-200a表达及与预后的关系.方法 选取郑州人民医院及郑州大学附属肿瘤医院肝胆外科行手术治疗的肝细胞肝癌患者95例,利用实时定量聚合酶链反应（Real-time PCR）技术检测肝癌患者血清及肿瘤组织中miR-200a表达,并分析miR-200a表达与临床病理特征及患者预后的关系.结果 肝癌患者血清中miR-200a相对表达量为4.57±1.36,低于对照组的5.98±0.62,差异有统计学意义（P＜0.05）;肝癌患者正常肝组织、癌旁组织及肝癌组织中miR-200a表达量分别为2.67 ±0.31、1.73±0.15和0.68 ±0.11,差异有统计学意义（P＜0.05）,两两比较,miR-200a表达量：肝癌组织＜癌旁组织＜正常肝组织,差异均有统计学意义（P＜0.05）;肝癌患者血清中miR-200a表达量与甲胎蛋白（AFP）水平、分化程度、是否转移、TNM分期有关（P＜0.05）,肝癌患者肝癌组织中miR-200a表达量与分化程度和是否转移有关（P＜0.05）;高表达组5年累积生存率为63.7％,低表达组5年累积生存率为41.9％,高表达组患者术后生存明显好于低表达组患者（x^2 =4.711,P＜0.05）.结论 miR-200a在肝癌患者血清和肝癌组织中均呈低表达,是重要的抑癌基因,与肿瘤恶性程度密切相关,并可影响患者预后.Objective To investigate the expressions of microRNA （miR）-200a in serum and carcinoma tissues and its relationship with prognosis in patients with hepatocellular carcinoma （HCC）.Methods Ninety-five cases of HCC undergoing surgical treatment in the Department of Hepatobiliary Surgery at Zhengzhou People＇ s Hospital and the Affiliated Tumor Hospital of Zhengzhou University were enrolled.The expression levels of miR-200a in serum and tumor tissues of patients with HCC were detected by using real-time quantitative polymerase chain reaction （Real-time PCR）.The relationships between the expression of miR-200a and clinicopathological features and prognosis of patients were analyzed.Results The relative expression of miR-200a in patients with HCC was 4.57 ± 1.36,statistically significantly lower than 5.98 ± 0.62 in the control group （P 〈 0.05）.The relative expression levels of miR-200a in normal liver tissue,adjacent tissue and liver cancer tissue were 2.67 ±0.31,1.73 ±0.15 and 0.68 ± 0.11 with the differences being statistically significant （P 〈 0.05）.The pairwise comparison showed that miR-200a expression levels were in HCC tissues 〈 adjacent tissues 〈 normal liver tissue （P 〈 0.05）.The expression of miR-200a in serum of HCC patients serum was correlated with alpha fetal protein （AFP） levels,degree of differentiation,metastasis and TNM staging （P 〈 0.05）.The expression of miR-200a in HCC tissues was correlated with degree of differentiation and metastasis （P 〈 0.05）.The 5-year cumulative survival rate in high miR-200a expression group was 63.7%,and that in the low miR-200a expression group was 41.9%.The survival of HCC patients in high miR-200a expression group was significantly improved as ocmpared with that in low miR-200a expression group （x^2 =4.711,P 〈 0.05）.Conclusion miR-200a showed low expression in serum and liver cancer tissue of patients with HCC.It is an important tumor suppressor gene,closely related to the degree of malignancy and can affect

关 键 词：癌 肝细胞 微小RNA-200a 血清 预后 

分 类 号：R735.7[医药卫生—肿瘤]