非小细胞肺癌易感性及预后与瘦素基因-2548G/A多态性的关系  

Association of Leptin gene-2 548 G/A polymorphism with the susceptibility and prognosis of non-small cell lung cancer

作　　者：曹凤军[1] 万国兴[1] 俞远东[1] 喻雄杰[1] 狄全书[1] 雷金华[1] 邓守恒[1] 陈萍[1] 

机构地区：[1]湖北医药学院附属人民医院肿瘤科,十堰442000

出　　处：《中华实验外科杂志》2015年第3期621-623,共3页Chinese Journal of Experimental Surgery

摘　　要：目的 探讨瘦素（Leptin）基因-2 548 G/A多态性位点与非小细胞肺癌易感性及预后的关系.方法 采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）技术检测162例非小细胞肺癌和200例正常者的基因型,收集整理病例的临床病理及预后资料,并进行统计学分析.结果 瘦素基因-2 548 G/A位点实验组中AA、GA基因型及A等位基因频率（AA：35.2％;GA：46.3％;A：58.3％）均分别高于对照组（AA：16.5％;GA：40.0％;A：41.7％）,差异有统计学意义[比值比（OR）=5.009,95％可信区间（CI）：2.758 -9.097,P＜0.01;OR=2.719,95％ CI：1.615-4.578,P＜0.01;OR=2.436,95％ CI：1.804 - 3.289,P＜0.01].Ⅲ＋Ⅳ期的非小细胞肺癌中基因型为GA＋AA的频率（58.3％）明显高于GG基因型（33.3％）,同样在淋巴结转移患者中基因型为GA＋ AA的频率（62.1％）明显高于GG基因型（40.0％）,差异均有统计学意义（P＜0.05）.基因型为GA＋ AA患者生存期[（37.7±1.1）个月]明显差于AA型患者[（45.9±2.9）个月],差异有统计学意义（x^2=8.215,P＜0.01）.结论 瘦素基因-2 548 G/A多态性可能与非小细胞肺癌易感性有关,并且可能是非小细胞肺癌的不良预后因子.Objective To explore the association of Leptin gene-2 548 G/A polymorphism with the susceptibility and prognosis of non-small cell lung cancer （NSCLC）.Methods A total of 162 patients with NSCLC and 200 controls were enrolled in the case-control study.The genotypes were detected using reaction-restriction fragment length polymorphism （PCR-RFLP）.Relevant clinicopathological data of selected cases were collected and analyzed.Results The frequencies of AA,GA and A allele in cases （AA：35.2 % ; GA：46.3 % ; A：58.3 %） were higher than that in controls （AA：16.5 % ; GA：40.0% ;A：41.7%）,and significant differences were revealed [odds ratio （OR） =5.009,95% confidence interval （CI）：2.758-9.097,P〈0.01;OR=2.719,95%CI：1.615-4.578,P〈0.01;OR=2.436,95%CI：1.804-3.289,P 〈 0.01],respectively.The frequencies of AA ＋ GA genotype （58.3%） was found higher than GG genotype（33.3%） in NSCLC in Ⅲ ＋ Ⅳ stage,likewise,the frequencies of AA ＋ GA genotype（62.1%） were also found higher than GG genotype（40.0%） in NSCLC with lymph node metastasis,significant differences were also showed （both P 〈 0.05）,and the survival of individuals carrying GA ＋ AA （37.7 ± 1.1） genotype was markedly reduced compared to that carrying GG （45.9 ± 2.9） genotype （x2 =8.215,P 〈 0.01）.Conclusion The present study suggests that the Leptin gene-2 548 G/A polymorphism may be associated with the susceptibility to NSCLC,and appear to jointly contribute to predicting a poor prognosis.

关 键 词：非小细胞肺癌 瘦素 基因多态性 

分 类 号：R734.2[医药卫生—肿瘤]