微小RNA-1260b在非小细胞肺癌组织的表达及临床意义  

Expression of microRNA-1260b in non-small-cell lung carcinoma and the clinical significance

作　　者：李静[1] 李丽琴[1] 许丽敏[1] 沈琦斌[2] 李鸿伟[2] 平金良[3] 马志红[1] 钟婧[1] 黄惠莲[1] 戴利成[1] 

机构地区：[1]湖州市中心医院湖州市分子医学重点实验室,浙江313000 [2]湖州市中心医院胸外科,浙江313000 [3]湖州市中心医院病理科,浙江313000

出　　处：《中华实验外科杂志》2015年第3期624-626,共3页Chinese Journal of Experimental Surgery

基　　金：浙江省自然科学基金重点资助项目（Z2101431）

摘　　要：目的 检测非小细胞肺癌（NSCLC）及癌旁组织中微小RNA（miRNA,miR）-1260b的表达,探讨miR-1260b在肺癌发生发展中的临床意义.方法 收集非小细胞肺癌组织和癌旁组织,通过miRNA芯片筛选出癌组织和癌旁组织之间的差异表达的miRNA,采用实时定量聚合酶链反应（Real-time PCR）进行扩大样本检测,验证差异miRNA在肺癌组织和癌旁组织的表达.结果 通过miRNA芯片实验筛选出差异表达的miRNA经扩大样本验证,采用循环阈值（ΔCt）对miR-1260b在肺癌和配对癌旁组织中的表达水平进行定量分析,ΔCt值分别为10.04±1.43和10.97±1.17;与对应的癌旁组织比较,82.2％的病例中miR-1260b表达升高（2-ΔΔCt值＞1）,只有18.8％的病例显示miR-1260b在肺癌组织的表达下降（2-ΔΔCt值＜1）,差异有统计学意义（P＜0.05）.此外,肺癌组织中miR-1260b的表达水平与患者年龄,性别、肿瘤分化程度和临床分期无明显正相关.结论 miR-1260b在非小细胞肺癌组织的表达水平高于对应的癌旁组织,可能与非小细胞肺癌的发生发展相关.Objective To investigate the expression of microRNA （miRNA,miR）-1260b in non-small-cell lung carcinoma （NSCLC） and paracarcinoma tissues and the clinical significance.Methods Seventy-eight pairs of lung cancer and paracarcinoma tissues were collected from patients sufferred from NSCLC.MicroRNA microarray was used to screen the miRNA with different expression levels in lung cancer and paracarcinoma tissues.The real-time quantitative polymerase chain reaction （Real-time PCR） was performed to detect the expression of target miRNA with enlarged clinical samples.The clinical analysis was carried out to explore the relationship between miR-1260b and clinical features.Results The ΔCt values of miR-1260b expression in lung cancer and paracarcinoma tissues were 10.04 ± 1.43 and 10.97 ± 1.17,respectively.Compared to paracarcinoma tissues,82.2% cases showed increased expression of miR-1260b in lung cancer tissues （P 〈 0.05）.Clinical analysis demonstrated that there was no significant correlation between the expression level of miR-1260b with sex,age,differentiation degree and TNM classification,respectively.Conclusion These results suggested that miR-1260b expression in lung cancer was higher than in paracarcinoma tissues,and may play important roles in the development and progression of lung cancer.

关 键 词：微小RNA-1260b 非小细胞肺癌 

分 类 号：R734.2[医药卫生—肿瘤]