维生素D对胃癌的作用及其机制研究  

Effects of vitamin D on gastric cancer and its underlying mechanisms

作　　者：缪玉娥[1] 刘海燕[1] 衣启君 王树超[1] 贾向磊[1] 王潍博[2] 林琦[2] 

机构地区：[1] 泰山医学院附属医院肿瘤科,泰安271000 [2] 山东省立医院肿瘤科,济南250021

出　　处：《国际生物医学工程杂志》2015年第1期43-47,I0006,共6页International Journal of Biomedical Engineering

摘　　要：目的 通过检测胃癌患者血清中维生素D（VD）含量及癌组织中维生素D受体（VDR）的表达水平,探讨VD对胃癌的作用及其机制.方法 采用酶联免疫吸附测定法（ELISA）测定胃癌患者及健康人群血清中的VD水平;采用免疫组化法检测胃癌组织及癌旁正常胃组织中的VDR表达水平,并分析其与预后的关系.结果 胃癌患者的血清VD水平较健康人群低（P＜0.05）,且与胃癌细胞分化程度明显负相关（P＜0.001）;胃癌组织中VDR表达显著低于正常胃黏膜（P＜0.05）,且VDR的表达水平与癌组织分化程度之间有显著联系,高、中、低分化3组VDR的表达水平依次降低且差异有统计学意义（P＜0.05）;胃癌患者中,VDR表达阳性者的无疾病进展生存期和总生存期均较VDR阴性者明显延长,差异均有统计学意义（P＜0.05）.结论 VD可能是胃癌发病中的一个保护性因素,其VDR的表达水平可作为判断胃癌分化程度的依据之一,VDR可作为胃癌术后的一个有效预后因子.Objective To investigate the effect of vitamin D （VD） on gastric cancer and its underlying mechanisms by detecting serum VD levels in gastric cancer patients and the expression of vitamin D receptor （VDR） in gastric tumor.Methods Serum VD levels was detected by enzyme linked immunosorbent assay,VRD expression of tumor tissue and normal mucosa were detected by immunohistochemistry.At the same time,relationships of VDR expression and the prognosis of the patients were analyzed.Results The serum levels of VD of gastric cancer patients were lower than that of healthy people （P〈0.05）,and they were negatively related to the degree of cell differentiation significantly （P〈0.001）.VDR expression in gastric tumor tissue significantly decreased compared to that of the normal mucosa （P〈0.05）.A significant correlation was found between the VDR expression and the differentiation grade of the carcinoma,with well differentiated carcinoma having the highest level of VDR expression （P〈0.05）.For patients with gastric cancer,those with positive VDR expression had significant longer progression-free survival （PFS） and overall survival （OS） than the patients with negative VDR expression （P〈0.05）.Conclusions VD may be a protecting factor of gastric cancer.VDR can be regarded as a marker of differentiation of gastric cancer and served as an effective prognostic factor in patients with postoperative gastric cancer.

关 键 词：维生素D 胃肿瘤 维生素D受体 

分 类 号：R735.2[医药卫生—肿瘤] Q565[医药卫生—临床医学]